Related Experiment Video

Updated: Feb 11, 2026

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

7.4K

Characterization and epitope mapping of a panel of monoclonal antibodies against HIV-1 matrix protein

Zhiqing Zhang1, Feng Zhang1, Shimeng Bai2

  • 1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, People's Republic of China.

Biotechnology and Applied Biochemistry
|April 18, 2018
PubMed

Insights

Researchers developed new monoclonal antibodies to track Human Immunodeficiency Virus type 1 (HIV-1) matrix (MA) protein conformations during virus assembly and maturation, aiding in understanding HIV-1 structure and potential therapies.

Area of Science:

  • Virology
  • Structural Biology
  • Immunology

Background:

  • The Human Immunodeficiency Virus type 1 (HIV-1) Gag precursor protein (p55) is essential for virion assembly.
  • The matrix (MA/p17) protein within Gag is crucial for membrane targeting and Env uptake, and may also be involved in uncoating and nuclear import.
  • MA exhibits reversible membrane-binding and conformational changes, but these are poorly understood during assembly.

Purpose of the Study:

  • To characterize monoclonal antibodies against MA with differential reactivity to various Gag precursor forms.
  • To investigate the potential of these antibodies in tracking MA conformations throughout the HIV-1 life cycle.
  • To explore the utility of these antibodies in structural determination and clinical applications.

Main Methods:

  • Characterization of a panel of monoclonal antibodies targeting the HIV-1 MA protein.
  • Assessment of antibody reactivity against different forms of the Gag precursor protein (p55, p41, p17).

Main Results:

  • A panel of monoclonal antibodies demonstrated distinct reactivity patterns towards p55, p41, and mature p17 forms of the MA protein.
  • These antibodies can differentiate between various conformations of MA present during HIV-1 assembly and maturation.

Conclusions:

  • The developed monoclonal antibodies can serve as tools to track MA conformations during the HIV-1 life cycle.
  • These antibodies may aid in determining the structure of MA and its precursors.
  • Potential clinical applications include therapeutic strategies against AIDS progression and diagnostic reagents for p17 detection.
Keywords:
HIV-1 matrix proteinmonoclonal antibodyp17

More Related Videos

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

10.1K
Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

14.7K

Related Concept Videos

Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
6.0K
Golgi Matrix Proteins01:12

Golgi Matrix Proteins

Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
2.5K
Wood Panel Products01:18

Wood Panel Products

Wood panel products are essential materials used in construction for applications such as flooring, siding, and roofing, typically available in standard dimensions of 4 feet by 8 feet, with thicknesses varying from one-quarter of an inch to one and one-eighth inches. Among the most common types of wood panels is plywood, which is produced by gluing multiple layers of thin wood veneers under pressure. The grain of the outer veneers runs lengthwise, while the grains of the interior layers run...
411
The Extracellular Matrix01:42

The Extracellular Matrix

Overview
89.4K
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.4K
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
65.7K