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Photodeposition of Pd onto Colloidal Au Nanorods by Surface Plasmon Excitation
Published on: August 15, 2019
Nanorod diameter modulated osteogenic activity of hierarchical micropore/nanorod-patterned coatings via a
Jianhong Zhou1, Lingzhou Zhao2, Bo Li3
1State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, China; Institute of Physics & Optoelectronics Technology, Baoji University of Arts and Sciences, Baoji, China.
Abstract:
Hierarchical micropore/nanorod-patterned strontium doped hydroxyapatite (Ca9Sr1(PO4)6(OH)2, Sr1-HA) structures (MNRs) with different nanorod diameters of about 30, 70 and 150 nm were coated on titanium, to investigate the effect of nanorod diameter on osteogenesis and the involved mechanism. Compared to micropore/nanogranule-patterned Sr1-HA coating (MNG), MNRs gave rise to dramatically enhanced in vitro mesenchymal stem cell functions including osteogenic differentiation in the absence of osteogenic supplements and in vivo osseointegration related to the nanorod diameter with about 70 nm displaying the best effects. MNRs activated the cellular Wnt/β-catenin pathway by increasing the expression of Wnt3a and LRP6 and decreasing the expression of Wnt/β-catenin pathway antagonists (sFRP1, sFRP2, Dkk1 and Dkk2). The exogenous Wnt3a significantly enhanced the β-catenin signaling activation and cell differentiation on MNG, and the exogenous Dkk1 attenuated the enhancing effect of MNRs on them. The data demonstrate that MNRs favor osseointegration via a Wnt/β-catenin pathway.
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