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Optimizing photon dose and frequency to enhance lipid productivity of thermophilic algae for biofuel production
Said Abu-Ghosh1, Zvy Dubinsky2, Gabi Banet3
1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel; Bar Ilan Institute of Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat-Gan 5290002, Israel.
Abstract:
The aim of this work was to examine the potential of the thermophilic green microalga Micractinium sp. to accumulate triacylglycerols (TAGs) and to develop a light strategy to increase TAG productivity in this alga. To this end, dense cultures of Micractinium sp. were grown at 37 °C under nitrogen (N) starvation and exposed to a light intensity of 1000 µmol photons m-2 s-1 of different light regimes. The highest per-biomass TAG-content and maximal volumetric productivities of TAG were displayed by the cultures grown under flashing light of 5 Hz with 50% duty cycle. Based on the results, a sufficiently high-starting culture density should be combined with a high irradiance delivered by an appropriate light regime to enhance the production of biomass enriched TAGs.
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