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A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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Dietary Mannan Oligosaccharides Modulate Gut Microbiota, Increase Fecal Bile Acid Excretion, and Decrease Plasma

Lisa R Hoving1,2, Saeed Katiraei1,2, Marieke Heijink3

  • 1Department of Human Genetics, Leiden University Medical Center, Leiden, 2300 RC, The Netherlands.

Molecular Nutrition & Food Research
|April 18, 2018
PubMed
Summary

Dietary Mannan oligosaccharides (MOS) significantly reduced atherosclerosis in mice by lowering cholesterol. This effect is linked to changes in gut microbiota, increased butyrate, and altered bile acid excretion.

Keywords:
atherosclerosischolesterolmannan oligosaccharidesmicrobiotashort-chain fatty acids

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Area of Science:

  • Nutritional science
  • Gastroenterology
  • Cardiovascular research

Background:

  • Mannan oligosaccharides (MOS) improve growth and reduce hyperlipidemia and inflammation.
  • Atherosclerosis is exacerbated by hyperlipidemia and inflammation.

Purpose of the Study:

  • To investigate the impact of dietary MOS on atherosclerosis progression.
  • To utilize the hyperlipidemic ApoE*3-Leiden.CETP (E3L.CETP) mouse model, which mimics human lipoprotein metabolism.

Main Methods:

  • Female E3L.CETP mice were fed a high-cholesterol diet with or without 1% MOS for 14 weeks.
  • Atherosclerotic lesions were quantified.
  • Blood markers, gut microbiota composition (16S rRNA sequencing), and fecal/cecal metabolites (GC-MS) were analyzed.

Main Results:

  • MOS reduced atherosclerotic lesions by up to 54% in the aortic root valve area.
  • MOS increased the abundance of cecal Bacteroides ovatus.
  • MOS increased cecal butyrate and fecal bile acids, without affecting blood lipids or cholesterol excretion.

Conclusions:

  • Dietary MOS mitigates atherosclerosis development in hyperlipidemic mice.
  • MOS lowers plasma cholesterol levels, likely mediated by gut microbiota interactions.
  • Increased cecal butyrate and fecal bile acid excretion are associated with MOS intervention.