Related Experiment Video
Updated: Feb 11, 2026
08:31
Murine Model of Leukemia Relapse to Induction Chemotherapy for Acute Lymphoblastic Leukemia
Published on: October 17, 2025
709
[A Modified Protocol for Rapid Establishing Mouse Model with Ph+ Positive Acute Lymphoblastic Leukemia]
Na Qi1, Xue Wang2, Qing-Yun Wu2
1Institute of Hematolog, Xuzhou Medical University, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, Jiangsu Province China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|April 19, 2018
Summary
Researchers developed a new, more effective mouse model for Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL). This improved model offers a simpler and more powerful tool for studying Ph+ ALL.
Area of Science:
- Hematology
- Oncology
- Animal Models
Background:
- Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) is a distinct subtype requiring effective research models.
- Existing mouse models for Ph+ ALL may present limitations in convenience and efficacy for comprehensive studies.
Purpose of the Study:
- To establish a modified, more convenient, and powerful mouse model for Ph+ ALL research.
- To provide an improved tool for investigating the pathogenesis and potential therapies of Ph+ ALL.
Main Methods:
- Immature B cells from BALB/c mice were retrovirally transfected with Mig190.
- Transfected cells were infused into irradiated syngeneic mice.
- Leukemia development was confirmed via immunophenotyping (flow cytometry), BCR-ABL detection (RT-PCR, Western blot), and subsequent re-infusion without irradiation.
Main Results:
- Successful establishment of Ph+ ALL in mice characterized by CD19+ lymphoblasts and BCR-ABL expression.
- Mig190 retroviral transfection induced acute lymphoblastic leukemia.
- Leukemia cells from affected mice rapidly induced B-ALL upon secondary transfer, confirming model efficacy.
Conclusions:
- A novel and effective protocol for generating a Ph+ ALL mouse model has been established.
- This new model offers a simpler and more potent alternative to conventional methods for Ph+ ALL research.
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