[Expression of MiR-296-5p in Diffuse Large B-Cell Lymphoma and Its Influence on Biological Behavior of Tumor Cells]
Related Experiment Video
Updated: Feb 11, 2026

Bioprinting of Hydrogel Tumor Slices as a 3D Model for Mantle Cell Lymphoma
Published on: September 12, 2025
Xiao-Si Liu1, Xin-Chen Wang1, Kai-Yang Ding1
1Department of Hematology, Provincial Hospital Affiliated to Anhui Medical University, Hefei 230000, Anhui Province, China.
Objective:
To detect the expression of miRNA-296-5p in diffuse large B cell lymphoma (DLBCL) cells and study the proliferation, migration and apoptosis of DLBCL cells after interfering the expression of miRNA-296-5p.
Methods:
The expression of miRNA-296-5p in DLBCL cells line DB cells was detected by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The possible roles of miRNA-296-5p in the biological and behavioral properties of DLBCL-DB cells were explored by transfection of miRNA-296-5p inhibitor for miRNA-296-5p knockdown, and detected by using CCK-8 method, Transwell method and Annexin V-FITC/PI.
Results:
miRNA-296-5p was highly expressed in DLBCL-DB cells, and the inhibition of miRNA-296-5p could induce suppression of cell proliferation and migration, but the cell apoptosis was not changed significantly.
Conclusion:
The high expression of miRNA-296-5p may relate with the occurence and development of DLBCL, which may be a new therapeutic target for DLBCL.
Related Concept Videos
Causes of Social Behavior III: Biological and Environmental Influences
Biological Influences on Intelligence
Behavior of Gas Molecules: Molecular Diffusion, Mean Free Path, and Effusion
Cell Specific Gene Expression
Cell Specific Gene Expression
Diffusion

