Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 12, 2026

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Published on: August 17, 2011

Radioreceptor assay for atrial natriuretic factor.

J Gutkowska¹, F Carrier, J St-Louis

¹Laboratory of Biochemistry of Hypertension, Clinical Research Institute of Montreal, Quebec, Canada.

Analytical Biochemistry

|January 1, 1988

Summary

We developed a rapid and sensitive radioreceptor assay (RRA) to measure atrial natriuretic factor (ANF). This new assay accurately measures ANF in biological samples, correlating well with established methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

"I want nothing to do with hospitals anymore": challenges, met and unmet needs of people experiencing homelessness with acquired brain injury.

Brain injury·2026

Same author

"Overnight, everything changed": life courses and turning points toward homelessness among people with acquired brain injury.

Brain injury·2026

Same author

Effects of submaximal and supramaximal interval training on determinants of endurance performance in endurance athletes.

Scandinavian journal of medicine & science in sports·2016

Same author

[N-methyl-D-aspartate receptor antibody encephalitis: value of immunomodulatory therapy].

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2014

Same author

Reply: To PMID 23918413.

Arthritis & rheumatology (Hoboken, N.J.)·2014

Same author

Rituximab-induced T cell depletion in patients with rheumatoid arthritis: association with clinical response.

Arthritis and rheumatism·2013

Area of Science:

Endocrinology
Physiology
Biochemistry

Background:

Atrial natriuretic factor (ANF) plays a role in salt and water homeostasis.
Accurate measurement of ANF is crucial for understanding its physiological functions.
High-affinity ANF binding sites exist in rat glomeruli.

Purpose of the Study:

To develop a rapid, sensitive, and simple radioreceptor assay (RRA) for measuring ANF.
To characterize the ANF binding site in rat glomeruli.
To validate the RRA by comparing it with radioimmunoassay (RIA).

Main Methods:

Utilized rat glomerular membranes as a source of ANF binding sites.
Incubated radioiodinated ANF with plasma extracts or atrial homogenates and glomerular membranes.

More Related Videos

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

Related Experiment Videos

Last Updated: Jul 12, 2026

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

Published on: August 17, 2011

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors

Published on: June 7, 2016

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

Separated bound and free radioactivity via filtration and measured using a scintillation counter.

Characterized binding kinetics including dissociation constant and binding capacity.

Main Results:

Characterized a high-affinity, saturable ANF binding site in rat glomeruli (Kd=33 pM, Bmax=396 fmol/mg).
The RRA demonstrated high sensitivity (2.08 fmol) and low intra-/interassay variability (<11%).
RRA results showed high correlation with RIA for plasma extracts (r=0.973) and atrial homogenates (r=0.954).

Conclusions:

The developed RRA is a reliable and sensitive method for quantifying ANF.
The assay's high correlation with RIA suggests measured ANF possesses biological activity.
This RRA facilitates further research into ANF's role in salt and water balance.