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[Gene expression of D-beta-hydroxybutyrate dehydrogenase. III. Molecular cloning of a DNAc]
A Bailly1, A Kanté, M Cherkaoui-Malki
1Laboratoire de Biochimie, UA CNRS 531 Biologie Moléculaire des Membranes, Université de Franche-Comté, Besancon, France.
Abstract:
In order to continue the molecular studies of D-beta-hydroxybutyrate dehydrogenase (BDH) undertaken in our laboratory for several years, we have initiated a genetic approach which consists in the BDH cDNA cloning from a rat liver cDNA library. The immunoscreening method allowed to isolate a clone which exhibits a DNA insert shorter than the expected full length BDH cDNA.