Expression of concern: ZnO nanoparticles affect intestinal function in an in vitro model

Area of Science:

• Nanotechnology
• Toxicology
• Gastroenterology

Background:

• Zinc oxide nanoparticles (ZnO NPs) are increasingly used in consumer products.
• Their potential impact on human health, particularly the gastrointestinal tract, requires thorough investigation.
• Understanding the interaction between nanomaterials and intestinal cells is crucial for risk assessment.

Purpose of the Study:

• To investigate the effects of ZnO NPs on intestinal epithelial cells in vitro.
• To assess the impact of ZnO NPs on intestinal barrier integrity and cellular functions.
• To evaluate the potential toxicity of ZnO NPs to the intestinal lining.

Main Methods:

• Utilized an in vitro intestinal model using Caco-2 cell monolayers.
• Exposed cells to varying concentrations of ZnO NPs.
• Assessed cell viability, barrier permeability (transepithelial electrical resistance), and inflammatory markers.

Main Results:

• ZnO NPs significantly reduced cell viability in a dose-dependent manner.
• Exposure to ZnO NPs increased intestinal permeability, indicating compromised barrier function.
• Evidence of inflammatory responses and oxidative stress was observed in treated cells.

Conclusions:

• ZnO NPs can adversely affect intestinal epithelial cells and compromise the intestinal barrier.
• These findings highlight potential risks associated with ZnO NP exposure through the gastrointestinal tract.
• Further in vivo studies are warranted to confirm these in vitro observations and assess overall health risks.