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Long non-coding RNA SNHG14 contributes to gastric cancer development through targeting miR-145/SOX9 axis

Zhao Liu1, Yan Yan2,3, Sizhe Cao4

  • 1Department of Surgery, Xi'an Chest Hospital, Xi'an TB&Thoracic Tumor Hospital, Xi'an, Shanxi, China.

Journal of Cellular Biochemistry
|April 19, 2018
PubMed

Insights

Long non-coding RNA SNHG14 promotes gastric cancer by targeting the miR-145/SOX9 axis and PI3K/AKT/mTOR pathway. Inhibiting SNHG14 offers a potential therapeutic strategy for gastric cancer treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Gastric cancer remains a significant global health challenge.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer progression.
  • The specific involvement of SNHG14 in gastric cancer pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the functional role of lncRNA SNHG14 in gastric cancer.
  • To identify the molecular mechanisms underlying SNHG14's contribution to gastric cancer development.
  • To explore the potential of targeting the SNHG14 pathway for gastric cancer therapy.

Main Methods:

  • Quantitative real-time PCR to assess SNHG14 expression levels.
  • Cell viability, migration, invasion, and apoptosis assays in gastric cancer cells.
  • Western blotting to analyze protein expression related to apoptosis, EMT, and the PI3K/AKT/mTOR pathway.
  • Luciferase reporter assays to confirm interactions between SNHG14, miR-145, and SOX9.

Main Results:

  • SNHG14 was significantly upregulated in gastric cancer tissues and cells.
  • Knockdown of SNHG14 inhibited cell viability, migration, and invasion, while promoting apoptosis.
  • SNHG14 negatively regulated miR-145, which in turn targeted SOX9, influencing the PI3K/AKT/mTOR pathway.

Conclusions:

  • Up-regulation of lncRNA SNHG14 promotes gastric cancer development.
  • The SNHG14/miR-145/SOX9 axis plays a crucial role in gastric cancer progression.
  • Targeting the SNHG14-miR-145/SOX9 axis represents a potential therapeutic strategy for gastric cancer.
Keywords:
PI3K/AKT/mTOR pathwaySNHG14SOX9gastric cancerlong non-coding RNAmiR-145

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