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Benzyl isothiocyanate attenuates the hydrogen peroxide-induced interleukin-13 expression through glutathione

Yue Tang1,2, Sho Naito2, Naomi Abe-Kanoh2,3

  • 1School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China.

Journal of Biochemical and Molecular Toxicology
|April 19, 2018
PubMed

We investigated the effect of benzyl isothiocyanate (BITC) on the hydrogen peroxide-induced gene expression of a T-helper-2 cytokine, interleukin (IL)-13, in T lymphocytic leukemia Jurkat cells. The 24-h pretreatment of BITC significantly inhibited the IL-13 expression enhanced by hydrogen peroxide. Although the BITC pretreatment did not change the enhanced level of the phosphorylated c-Jun N-terminal kinase (JNK), it significantly inhibited the nuclear translocation of c-Jun induced by hydrogen peroxide. BITC also increased the protein expression of glutathione S-transferase (GST) isozymes, GSTP1/2, as well as the total GST activity. A GSTP1/2-specific inhibitor, 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX), significantly counteracted the inhibitory effect of BITC on the hydrogen peroxide-enhanced IL-13 upregulation as well as the c-Jun nuclear translocation. Taken together, these results suggested that BITC inhibits the oxidative stress-mediated IL-13 mRNA expression, possibly through interference of the c-Jun phosphorylation by GSTP.

Keywords:
benzyl isothiocyanatec-Junglutathione S-transferasehydrogen peroxideinterleukin 13

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