Development and Validation of HPLC Analytical Method for Chlorantraniliprole in Bulk and in the Formulation

Chlorantraniliprole is widely used as a pesticide. It is only soluble in dimethyl formamide. However, most of the reported methods used acetonitrile and other solvents.

To develop rapid, accurate, precise, and sensitive HPLC method for chlorantraniliprole.

10 μG/mL of chlorantraniliprole containing solution was injected into the HPLC system and run in different solvent systems. 10 μG/mL solutions of chlorantraniprole was injected in a column with 20 μL microsyringe. The chromatogram was run for appropriate minutes with mobile phase water. The flow rate was set to 1 mL/min and detection was carried out at wavelength 270 nm. The method is validated by measuring the limit of quantitation, limit of detection, repeatability, intraday precision, inter-day precision, and accuracy. Analysis of marketed formulation, bringle, and tomato for chlorantraniprole content was also made for the developed analytical method.

There was linearity of chlorantraniliprole for calibration curve in between 1 and 5 μG/mL concentration. The perfect sharp peak observed in water at a retention time of 6.28 min. The limit of detection and the limit of quantitation were 0.0050 μG/mL and 0.0152 μG/mL, respectively. The study reported 99.55% repeatability, 99.49% intraday precision, 99.65% interday precision, and 99.27% accuracy.

The rapid, accurate, precise, and sensitive HPLC method for the detection of chlorantraniliprole using dimethyl formamide was developed.