Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PARN and TOE1 Constitute a 3' End Maturation Module for Nuclear Non-coding RNAs.

Ahyeon Son1, Jong-Eun Park2, V Narry Kim1

  • 1Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Korea.

Cell Reports
|April 19, 2018
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of the SARS-CoV-2 genome packaging signal in the nsp12-coding region.

Nature communications·2026
Same author

Comparative Analysis of Meat Quality and Muscle Transcriptome between Landrace and Jeju Native Pig.

Food science of animal resources·2026
Same author

Glutamate excreted by LepR⁺ BM-MSCs mitigates alcohol-associated liver disease by promoting IL-1R2⁺ monocyte migration.

Clinical and molecular hepatology·2026
Same author

Longitudinal profiling reveals immune dynamics and distinct plasma cell signatures during B-cell depletion in IgG4-related disease.

Annals of the rheumatic diseases·2026
Same author

Structural basis for chaperone-guided assembly of RNA-induced silencing complex.

Nature·2026
Same author

Orbital-Hybridizable Nanoseed Interphase Enables One-Minute Rechargeable, Energy-Dense Anode-Free Aqueous Zinc Batteries.

Advanced materials (Deerfield Beach, Fla.)·2026

Poly(A)-specific ribonuclease (PARN) and target of EGR1 protein 1 (TOE1) are crucial for nuclear small non-coding RNA maturation, not mRNA poly(A) tail length. Their redundant functions impact small Cajal body-specific RNAs and telomerase RNA biogenesis.

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Genetics

Background:

  • Poly(A)-specific ribonuclease (PARN) and target of EGR1 protein 1 (TOE1) are nuclear deadenylases implicated in human diseases.
  • Their precise molecular functions, particularly concerning non-coding RNAs, remain incompletely understood.

Purpose of the Study:

  • To systematically identify the substrates of PARN and TOE1 using advanced sequencing techniques.
  • To elucidate the molecular functions of PARN and TOE1 in nuclear RNA processing.
  • To understand the role of these enzymes in ncRNA biogenesis and associated human disorders.

Main Methods:

  • Application of mTAIL-seq and RNA sequencing (RNA-seq) to identify PARN and TOE1 substrates.
  • Comparative analysis of RNA populations under different PARN and TOE1 activity levels.
Keywords:
3′ end maturationCAF1ZPARNTERCTOE1adenylationdeadenylasedeadenylationscaRNA

Related Experiment Videos

Main Results:

  • PARN and TOE1 were found to promote the maturation of nuclear small non-coding RNAs (ncRNAs), rather than modulating mRNA poly(A) tail length.
  • PARN and TOE1 exhibit redundant roles in the biogenesis of small Cajal body-specific RNAs (scaRNAs) and telomerase RNA component (TERC).
  • Combined deficiency in PARN and TOE1 leads to downregulation of scaRNAs, causing defects in small nuclear RNA (snRNA) pseudouridylation.

Conclusions:

  • PARN and TOE1 are key regulators of nuclear ncRNA biogenesis, particularly scaRNAs and TERC.
  • Their redundant functions highlight a critical pathway for ncRNA maturation.
  • Findings offer insights into the molecular basis of PARN/TOE1-associated genetic disorders, potentially guiding future therapeutic strategies.