Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In Vitro Fertilization01:24

In Vitro Fertilization

1.0K
In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...
1.0K
Fertilization01:38

Fertilization

91.8K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
91.8K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

16.6K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
16.6K
Equivalence: In Vitro and In Vivo Bioequivalence01:17

Equivalence: In Vitro and In Vivo Bioequivalence

243
Body:Bioequivalence studies are crucial in evaluating whether new drugs can match an approved one regarding pharmacological effects and clinical performance. These studies test if drugs, despite different dosage forms, share identical plasma concentration-time profiles. Three types of equivalence are central to these studies: chemical, pharmaceutical, and therapeutic. Chemical equivalence indicates that two or more drug products contain identical active ingredients in equal amounts.
243
In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

251
Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
251
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

643
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
643

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stage- and Region-Dependent Proteomic Alterations in a Mouse Model of Creatine Transporter Deficiency.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Rat DCST1 and DCST2 are essential for sperm-egg fusion†.

Biology of reproduction·2026
Same author

Molecular and histological characterizations reveal two distinct senescent microglia populations in Niemann-Pick disease type C mouse model.

GeroScience·2026
Same author

Spiral ligament dysfunction and endocochlear potential loss drive hearing impairment in Niemann-Pick C1 mice.

Brain research bulletin·2026
Same author

Protective role of early Tnfsf15 upregulation in limiting glomerular injury and proteinuria in experimental Alport Syndrome.

Journal of pharmacological sciences·2026
Same author

α1-acid Glycoprotein mitigates MASLD progression by modulating liver and adipose tissue function.

Endocrinology·2026

Related Experiment Video

Updated: Feb 11, 2026

Production of Haploid Zebrafish Embryos by In Vitro Fertilization
07:54

Production of Haploid Zebrafish Embryos by In Vitro Fertilization

Published on: July 14, 2014

25.3K

In Vitro Fertilization in Mice.

Toru Takeo, Naomi Nakagata

    Cold Spring Harbor Protocols
    |April 20, 2018
    PubMed
    Summary
    This summary is machine-generated.

    This study enhances in vitro fertilization success by optimizing sperm and oocyte handling. Using methyl-β-cyclodextrin (MBCD) and specific fertilization media improves fertilization rates.

    More Related Videos

    Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
    10:52

    Gamete Collection and In Vitro Fertilization of Astyanax mexicanus

    Published on: May 25, 2019

    10.5K
    A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
    11:01

    A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

    Published on: July 6, 2009

    32.1K

    Related Experiment Videos

    Last Updated: Feb 11, 2026

    Production of Haploid Zebrafish Embryos by In Vitro Fertilization
    07:54

    Production of Haploid Zebrafish Embryos by In Vitro Fertilization

    Published on: July 14, 2014

    25.3K
    Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
    10:52

    Gamete Collection and In Vitro Fertilization of Astyanax mexicanus

    Published on: May 25, 2019

    10.5K
    A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
    11:01

    A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

    Published on: July 6, 2009

    32.1K

    Area of Science:

    • Reproductive biology
    • In vitro fertilization techniques

    Background:

    • In vitro fertilization (IVF) success is multifactorial, influenced by genetics, gamete handling, and culture environments.
    • Optimizing sperm and oocyte preparation is crucial for improving fertilization outcomes.

    Purpose of the Study:

    • To develop a protocol for enhanced in vitro fertilization (IVF) rates.
    • To investigate the impact of specific preincubation and fertilization media on fertilization success.

    Main Methods:

    • Sperm preincubation in medium supplemented with methyl-β-cyclodextrin (MBCD).
    • Fertilization using a medium characterized by high calcium and reduced glutathione (GSH) concentrations.

    Main Results:

    • The protocol demonstrated enhanced rates of fertilization.
    • Specific chemical modifications in sperm and fertilization media positively impacted IVF outcomes.

    Conclusions:

    • The described protocol offers a promising approach to improve IVF efficiency.
    • Optimized gamete handling and media composition are key factors for successful fertilization.