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Concepts in Light Microscopy of Viruses
Robert Witte1, Vardan Andriasyan2, Fanny Georgi3
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. robert.witte@uzh.ch.
Viruses
|April 20, 2018
Summary
Advanced light microscopy techniques, including fluorescence and super-resolution imaging, offer new ways to visualize viruses. This research guides the use of novel probes and methods to study virus infections and host interactions.
Area of Science:
- Virology
- Microscopy
- Cell Biology
Background:
- Viruses pose significant threats to humans, livestock, and plants, necessitating effective countermeasures.
- Understanding virus-host interactions is crucial for developing antiviral strategies and treatments.
- Light microscopy is essential for studying viral nature and infection dynamics.
Purpose of the Study:
- To provide an overview of recent light microscopy technologies for imaging cells and viruses.
- To guide the application of fluorescent probes and proteins in studying virus infections.
- To explore advanced microscopy techniques and their potential in virus research.
Main Methods:
- Review of fluorescence microscopy techniques (static and live-cell).
- Discussion of confocal, multi-photon, selective plane illumination, and super-resolution microscopy.
- Exploration of image processing and data analysis in microscopy.
Main Results:
- Novel fluorescent chemical probes and proteins enable detailed visualization of cells and viruses.
- Advanced microscopy methods offer enhanced resolution and imaging capabilities for virus studies.
- Established image processing techniques are vital for analyzing microscopy data.
Conclusions:
- Light microscopy, particularly advanced fluorescence and super-resolution techniques, is a powerful tool for virus research.
- Effective use of fluorescent probes and optimized imaging strategies are key to advancing our understanding of virus infections.
- Future directions include label-free microscopy for comprehensive virus visualization.
Keywords:
DNA virusRNA virusadeno-associated virus AAVadenovirusbaculoviruscell biologycomputingcytoskeletondata analysisendocytosisenveloped virusfluorescence microscopyfluorescent virionsgene expressiongene therapyhepatitis B virusherpes simplex virusherpesvirushigh-throughput screening, modelinghuman immunodeficiency virus HIVimage analysisimmunofluorescence microscopyinfectioninfluenza virusinnate immunityinternalizationintracellular transportlight microscopylive imagingmachine learningmembrane trafficmicroscopynonenveloped virusparvovirusquantitative microscopyreceptorsimian virus 40simulationsuper-resolutiontraffickingvirion uncoatingvirus entryvirus infectionvirus labeling
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