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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Transfecting RAW264.7 Cells with a Luciferase Reporter Gene
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Reporter Gene Assays Using Transfectable Functional Genomics Libraries.

Genevieve Welch1,2, Robert Damoiseaux3,4,5, Loren Miraglia6,7

  • 1Department of Genomics, The Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2018
PubMed
Summary
This summary is machine-generated.

Arrayed functional genomics libraries enable the study of cellular processes in health and disease. This overview covers generating, screening, and analyzing these powerful tools for biological discovery.

Keywords:
AutomationData analysisFunctional genomicsHTSHigh-throughput screeningLibrariesTransfectioncDNAmiRNAsiRNA

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Area of Science:

  • Genomics
  • Molecular Biology
  • Cell Biology

Background:

  • Functional genomics screening relies on reporter gene assays.
  • Arrayed libraries provide insights into cellular processes in health and disease.
  • These libraries are crucial for dissecting complex biological networks.

Purpose of the Study:

  • To provide an overview of principles for generating, screening, and analyzing arrayed functional genomics libraries.
  • To offer a comprehensive understanding of these technologies and their applications.

Main Methods:

  • Overview of transfectable reagent differences.
  • Guidance on library selection and handling.
  • Principles of data analysis for arrayed libraries.

Main Results:

  • Detailed explanation of arrayed library generation and screening.
  • Comprehensive guide to data analysis strategies.
  • Discussion of reagent choices and library management.

Conclusions:

  • Arrayed functional genomics libraries are essential for understanding cellular functions.
  • This work provides a foundational guide for applying these technologies.
  • Effective use of these libraries aids in deconvoluting complex disease phenotypes.