Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

9.2K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

3.5K
3.5K
Reporter Genes02:11

Reporter Genes

13.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.4K
Size and Structure of Viral Genomes01:26

Size and Structure of Viral Genomes

814
Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
814
Genomics02:02

Genomics

40.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
Viral Structure00:56

Viral Structure

74.8K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DUSP12 promotes cell cycle progression and protects cells from ZNF622 mediated apoptosis.

Cell death & disease·2026
Same author

Mechanistic Language Modeling and Oxygenated 3D Screening Reveal Berberine and Enzalutamide Synergy in Resistant Prostate Cancer.

bioRxiv : the preprint server for biology·2026
Same author

Phosphatidylcholine Metabolism Controls Alveolar Progenitor Renewal and Pulmonary Fibrosis.

bioRxiv : the preprint server for biology·2025
Same author

Orthogonal IMiD-Degron Pairs Induce Selective Protein Degradation in Cells.

ACS chemical biology·2025
Same author

Genomic copy-number variants drive apoptotic evasion underlying acquired resistance to immune checkpoint inhibitors.

Immunity·2025
Same author

Development of a leucine-rich repeat-containing protein 15-targeted radio-immunotheranostic approach to deplete pro-tumorigenic mechanisms and immunotherapy resistance.

Signal transduction and targeted therapy·2025

Related Experiment Video

Updated: Feb 11, 2026

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

11.9K

Reporter Gene Assays Using Viral Functional Genomics Libraries.

Genevieve Welch1,2, Robert Damoiseaux3,4,5, Loren Miraglia6,7

  • 1Department of Genomics, The Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2018
PubMed
Summary

Viral delivery systems offer a solution for introducing genomic reagents into hard-to-transfect cells, overcoming limitations of traditional transfection methods for cDNA, shDNA, miRNA, and guide RNA libraries.

Keywords:
CRISPRFunctional genomicsGain of functionLentiviralLibrary managementLoss of functionRetroviralcDNAmiRNAshRNA

More Related Videos

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.8K
Large Insert Environmental Genomic Library Production
20:59

Large Insert Environmental Genomic Library Production

Published on: September 23, 2009

16.4K

Related Experiment Videos

Last Updated: Feb 11, 2026

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
10:48

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Published on: May 15, 2014

11.9K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.8K
Large Insert Environmental Genomic Library Production
20:59

Large Insert Environmental Genomic Library Production

Published on: September 23, 2009

16.4K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Transfectable libraries are widely used in screening cores but are ineffective for hard-to-transfect cell lines and primary cells.
  • Viral delivery systems provide an alternative strategy for introducing genomic reagents into challenging cell types.

Purpose of the Study:

  • To detail the use of lentiviral and retroviral libraries for genomic reagent delivery.
  • To provide guidance on working with arrayed well formats for viral library production and application.

Main Methods:

  • Utilizing lentiviral and retroviral vectors for the introduction of genomic reagents.
  • Employing arrayed well formats for the organization and application of viral libraries.
  • Discussing the viral production step and necessary infrastructure for generating viral libraries.

Main Results:

  • Viral delivery enables the use of genomic libraries (cDNAs, shDNAs, miRNAs, guide RNAs) in previously untransfectable cells.
  • Established protocols for handling and utilizing lentiviral and retroviral libraries in arrayed formats.

Conclusions:

  • Viral-based genomic reagent delivery is a valuable and increasingly common technique for cell-based screening.
  • Understanding the specifics of viral production and arrayed formats is crucial for successful implementation.