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In vitro study evaluating the instantaneous treatment of ozonised olive oil on human sperm

Yan Fan1, Na Chen1, Mei Wang1

  • 1a Family Planning Research Institute , Tongji Medical College, Huazhong University of Science and Technology , Wuhan , P. R. China.

The European Journal of Contraception & Reproductive Health Care : the Official Journal of the European Society of Contraception
|April 20, 2018
PubMed
Abstract

Insights

Ozonised olive oil (OOO) demonstrates spermicidal potential, effectively immobilizing human sperm in vitro. This study suggests OOO may be a viable option for developing new vaginal contraceptive methods.

Area of Science:

  • Reproductive biology
  • Pharmacology

Background:

  • In vitro evaluation of novel spermicidal agents is crucial for contraceptive development.
  • Ozonised olive oil (OOO) has demonstrated various biological activities.

Purpose of the Study:

  • To assess the in vitro effects of ozonised olive oil (OOO) on human sperm parameters.
  • To determine the minimum effective concentration (MEC) of OOO for sperm immobilization.

Main Methods:

  • Human sperm were incubated with varying concentrations of OOO.
  • Sperm motility, viability, mitochondrial and acrosomal status, and DNA integrity were assessed.
  • Transmission electron microscopy was used to evaluate sperm ultrastructure.

Main Results:

  • OOO exhibited a dose-dependent inhibition of sperm motility.
  • The MEC of OOO for complete sperm immobilization within 20 seconds was determined to be 6 μg/ml.
  • Treatment with OOO significantly impacted sperm ultrastructure, function, and DNA integrity.

Conclusions:

  • Ozonised olive oil possesses significant spermicidal properties.
  • OOO shows potential as a novel vaginal contraceptive agent.
Keywords:
Acrosome reactionDNA integritycontraceptionozonised olive oilspermicide

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