[INFLUENCE OF COLD STRESS ON FUNCTIONAL ACTIVITY OF MOUSE PERITONEAL MACROPHAGES UNDER OPIATE RECEPTORS BLOCKADE]

The effect of 10- and 60-min cold stress was analyzed on reactive oxygen species (ROS) formation, peritoneal macrophage production of cytokines and corticosterone level under the opiate receptor blockade. It was observed that mice exposed to -20 °C for 10 min demonstrated the ROS inhibition, the effect was leveled against the background of naloxone introduction. The animal group subjected to 60-min stress on the contrary, showed the naloxone-dependent promotion of ROS product. Both variants of cold stress did not render significant effect on IL-1ß production. Against 60-min cold stress the TNF-α production was naloxone-dependently stimulated both in spontaneous and zymosan-induced cultures. IL-10 production by macrophages was improved irrespective of the cold stress duration, as well as stimulation presence or absence; and was abolished against a background of opiate receptor blockade. Therefore, the directionality of acute cold stress on the macrophage functions was dependent both on the parameters'' analyzed and on the duration of the exposure while the opiate receptor blockade significantly modified the immunoregulatory effects of hypothermia.