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Updated: Feb 11, 2026

Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril
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Cucurbit[7]uril-Dimethyllysine Recognition in a Model Protein.

Francesca Guagnini1,2, Paweł M Antonik1, Martin L Rennie1

  • 1School of Chemistry, National University of Ireland Galway, University Road, Galway, Ireland.

Angewandte Chemie (International Ed. in English)
|April 20, 2018
PubMed
Summary

Cucurbit[7]uril (Q7) fully encapsulates dimethyllysine (KMe2) in a model protein. This host-guest complexation, characterized by X-ray crystallography, reveals novel Q7-protein assemblies and potential strategies for controlled protein architecture.

Keywords:
cucurbiturildesolvationmacrocyclesmolecular gluessupramolecular chemistry

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Area of Science:

  • Supramolecular chemistry
  • Structural biology
  • Biophysical chemistry

Background:

  • Host-guest complexation is crucial in molecular recognition and drug delivery.
  • Cucurbiturils are macrocyclic hosts with a unique cavity structure.
  • Dimethyllysine is a post-translational modification found in proteins.

Purpose of the Study:

  • To structurally characterize the host-guest complexation between cucurbit[7]uril (Q7) and dimethyllysine (KMe2) in a model protein.
  • To investigate the binding modes and selectivity of Q7-KMe2 complexation.
  • To explore the formation of supramolecular assemblies between Q7, KMe2, and the protein.

Main Methods:

  • X-ray crystallography was employed to determine the high-resolution structures of the Q7-KMe2-protein complexes.
  • Solution-state studies were conducted to assess binding selectivity.
  • Analysis of crystal structures revealed details of encapsulation, solvation, and protein assembly.

Main Results:

  • Complete encapsulation of the dimethylammonium group of KMe2 within the Q7 cavity was observed.
  • Selective binding of Q7 to the most sterically accessible KMe2 residues was confirmed.
  • Three distinct Q7-KMe2 complexation modes and entrapped water molecules within the Q7 cavity were identified.
  • Novel Q7-protein assemblies, including inter-locked octahedral cages, were discovered in the solid state.

Conclusions:

  • Q7 effectively forms host-guest complexes with KMe2 within a protein environment.
  • The structural insights provide a basis for understanding KMe2 recognition by cucurbiturils.
  • The observed Q7-protein assemblies suggest a strategy for designing controlled protein architectures using cucurbituril clusters.