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Updated: Feb 11, 2026

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
Bio-Based Strategies for Producing Glycosaminoglycans and Their Oligosaccharides
Zhen Kang1, Zhengxiong Zhou2, Yang Wang2
1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Abstract:
Glycosaminoglycans (GAGs) and their oligosaccharides have attracted extensive attention because of their wide applications in cosmetics, health, and clinical treatment of, for example, thrombus, osteoarthritis, rheumatism, and cancer. Compared to conventional preparation approaches, bio-based strategies for producing GAGs and their oligosaccharides are more promising because they avoid cross-infection of human and animal diseases, use green production processes, and can precisely control the degree of sulfation and molecular weight. This review summarizes recently developed bio-based strategies for producing GAGs, highlighting in particular enzymatic, metabolic engineering, and synthetic biology strategies. Directions and prospects for near-future research are also outlined and discussed.
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