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Draft Genome Sequence of a Highly Resistant Streptococcus pneumoniae Serotype 15A Strain Isolated from Blood

Revathy Arushothy1, Norazah Ahmad2, Fairuz Amran2

  • 1Bacteriology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia revathy@imr.gov.my.

Genome Announcements
|April 21, 2018
PubMed

Insights

The emergence of antibiotic-resistant Streptococcus pneumoniae serotype 15A is a growing concern following vaccination. This study details the genome of a multidrug-resistant strain, SS40_16, highlighting its genetic makeup.

Area of Science:

  • Microbiology
  • Genomics
  • Vaccinology

Background:

  • The introduction of pneumococcal conjugate vaccines (PCVs) has reduced invasive pneumococcal disease.
  • However, PCVs can lead to the emergence of nonvaccine serotypes, including antibiotic-resistant strains.
  • Serotype 15A has shown a notable increase in prevalence.

Purpose of the Study:

  • To report the draft genome sequence of a multidrug-resistant Streptococcus pneumoniae serotype 15A strain.
  • To characterize the genetic basis of antibiotic resistance in this emerging serotype.

Main Methods:

  • Whole-genome sequencing of Streptococcus pneumoniae strain SS40_16.
  • Antimicrobial susceptibility testing to determine resistance profiles.
  • Serotyping and sequence typing for strain characterization.

Main Results:

  • The draft genome sequence of Streptococcus pneumoniae strain SS40_16 was obtained.
  • SS40_16 is a multidrug-resistant strain, exhibiting resistance to penicillin, tetracycline, and trimethoprim-sulfamethoxazole.
  • The strain was identified as serotype 15A and sequence type 1591 (ST1591).

Conclusions:

  • The characterization of this multidrug-resistant serotype 15A strain provides valuable genomic data.
  • This information is crucial for understanding the epidemiology and evolution of nonvaccine serotypes post-PCV introduction.
  • Genomic surveillance is essential to monitor and combat antibiotic-resistant Streptococcus pneumoniae.

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