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Related Experiment Video

Updated: Feb 11, 2026

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

A simple route to highly active single-enzyme nanogels.

Ana Beloqui^1,2, Andrei Yu Kobitski³, Gerd Ulrich Nienhaus^1,3,4,5

¹Institute of Toxicology and Genetics , Karlsruhe Institute of Technology (KIT) , Hermann-von-Helmholtz-Platz 1 , 76344 Eggenstein-Leopoldshafen , Germany . Email: guillaume.delaittre@kit.edu ;

Chemical Science

|April 21, 2018

Summary

We developed a simple one-step method to create single-enzyme nanogels (SENs) for enhanced enzyme stability. This approach preserves enzyme structure and activity across a wider pH range, ideal for biocatalysis.

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Published on: January 24, 2025

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Modeling an Enzyme Active Site using Molecular Visualization Freeware

Modeling an Enzyme Active Site using Molecular Visualization Freeware

Published on: December 25, 2021

Area of Science:

Biotechnology
Materials Science
Enzyme Engineering

Background:

Enzyme stability is crucial for biocatalysis but often limited.
Existing methods for enzyme stabilization can be complex and affect enzyme structure.

Purpose of the Study:

To develop a simple, one-step synthesis for single-enzyme nanogels (SENs).
To enhance enzyme stability and activity while preserving native enzyme structure.
To demonstrate the tunability of nanogel properties and their impact on enzyme performance.

Main Methods:

One-step synthesis of single-enzyme nanogels (SENs) using a small amount of sucrose.
Encapsulation of various enzymes within hydrophilic, crosslinked polymeric nanostructures.
Characterization of nanogel properties, including hydrogel layer thickness.
Single-molecule analysis using two-color confocal fluorescence coincidence analysis.

Main Results:

The one-step synthesis with sucrose is as effective as two-step methods, avoiding enzyme pre-modification.
Encapsulated enzymes retain high catalytic activity, similar to free enzymes, over a widened pH range.
Hydrogel shell thickness can be precisely controlled, significantly impacting enzyme activity and stability.
The method efficiently produces SENs, minimizing enzyme-free nanoparticles.

Conclusions:

The developed one-step SEN synthesis is a highly attractive method for biocatalysis.
This approach significantly enhances enzyme stability and activity while preserving native structure.
Tunable nanogel properties offer a versatile platform for optimizing biocatalytic applications.