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Published on: August 31, 2016
High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor
Ngong Kodiah Beyeh1,2, Isabel Díez1, S Maryamdokht Taimoory2
1Aalto University , School of Science , Department of Applied Physics , Puumiehenkuja 2 , FI-02150 , Espoo , Finland . Email: tbeyeh@gmail.com ;
Abstract:
Pyrophosphate (PPi) is a byproduct of DNA and RNA synthesis, and abnormal levels are indicative of disease. We report the high-affinity binding of PPi in water by N-alkyl ammonium resorcinarene chloride receptors. Experimental analysis using 1H and 31P NMR, isothermal titration calorimetry, mass spectrometry, and UV-vis spectroscopy all support exceptional selectivity of these systems for PPi in water. The measured affinity of K1 = 1.60 × 107 M-1 for PPi is three orders of magnitude larger than that observed for binding to another phosphate, ATP. This exceptional anion-binding affinity in water is explored through a detailed density functional theory computational study. These systems provide a promising avenue for the development of future innovative medical diagnostic tools.
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