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Free-Floating Mesothelial Cells in Pleural Fluid After Lung Surgery
Arne Kienzle1, Andrew B Servais1, Alexandra B Ysasi1
1Laboratory of Adaptive and Regenerative Biology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, United States.
Frontiers in Medicine
|April 21, 2018
Summary
Mesothelial cells are present in pleural fluid after lung surgery, peaking on post-operative day 3. These viable, activated cells may play a role in repairing surgical injury to the mesothelium.
Area of Science:
- Cell Biology
- Regenerative Medicine
- Surgical Research
Background:
- The mesothelium, a vital tissue layer, is involved in organ-specific diseases and regeneration.
- Mechanisms of mesothelial repair following surgical injury remain largely unknown.
- Free-floating mesothelial cells may contribute to healing by seeding denuded surfaces.
Purpose of the Study:
- To investigate the presence and characteristics of mesothelial cells in pleural fluid after pulmonary surgery.
- To determine the temporal dynamics of mesothelial cells in the early postoperative period.
Main Methods:
- Flow cytometry was used to analyze pleural fluid from 45 patients undergoing lung surgery.
- Mesothelial cells were identified using validated markers CD71 and WT1.
Main Results:
- Pleural fluid cells exhibited near 100% viability.
- Both leukocytes and mesothelial cells were detected within one week post-surgery.
- Mesothelial cells peaked on post-operative day 3, with CD71 expression indicating activation or proliferation.
Conclusions:
- Post-lung surgery pleural fluid contains viable, activated mesothelial cells.
- The peak concentration of mesothelial cells on post-operative day 3 suggests a potential role in mesothelial repair after pulmonary surgery.
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