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Updated: Feb 11, 2026

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
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Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies.

Gregory H Underhill1, Salman R Khetani2

  • 1Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois.

Cellular and Molecular Gastroenterology and Hepatology
|April 21, 2018
PubMed
Summary
This summary is machine-generated.

Advanced bioengineered liver models improve drug safety testing and disease modeling. These in vitro systems enhance primary human hepatocyte function for better drug development and cell-based therapies.

Keywords:
3D, 3-dimensionalBAL, bioartificial liverBioprintingCRP, C-reactive proteinCYP450, cytochrome P450Cellular MicroarraysDILI, drug-induced liver injuryECM, extracellular matrixHSC, hepatic stellate cellHepatocytesIL, interleukinKC, Kupffer cellLSEC, liver sinusoidal endothelial cellMPCC, micropatterned co-cultureMicrofluidicsMicropatterned Co-CulturesNPC, nonparenchymal cellPEG, polyethylene glycolPHH, primary human hepatocyteSpheroidsiHep, induced pluripotent stem cell-derived human hepatocyte-like celliPS, induced pluripotent stem

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Area of Science:

  • Biomedical Engineering
  • Hepatology
  • Drug Development

Background:

  • In vitro liver models are crucial for drug safety, disease modeling, and cell therapies.
  • Primary human hepatocytes (PHHs) in conventional cultures rapidly lose function.
  • Engineering approaches offer precise control over the cellular microenvironment.

Purpose of the Study:

  • To review advances in bioengineered in vitro human liver models.
  • To highlight utility in drug screening and understanding liver cell function determinants.
  • To discuss design features, validation, and future challenges.

Main Methods:

  • Utilizing engineering tools like microarrays, microfluidics, and bioprinting.
  • Co-culturing with nonparenchymal cells for long-term hepatocellular function.
  • Developing liver organoid culture systems for cell expansion.

Main Results:

  • Bioengineered models enhance PHH and stem cell-derived hepatocyte function.
  • Long-term stabilization (4+ weeks) of hepatocellular function achieved.
  • Liver organoids offer enhanced expansion of therapeutically relevant cells.

Conclusions:

  • Bioengineered liver models significantly advance understanding of liver function and injury.
  • These models are valuable for drug development and preclinical testing.
  • Future work should address pending issues for broader clinical application.