New STs in multidrug-resistant Acinetobacter baumannii harbouring β-lactamases encoding genes isolated from Brazilian
J P R Furlan1, A Pitondo-Silva1, E G Stehling1
1Department of Clinical Analysis, Toxicology and Food Science, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, Brazil.
Aims:
We investigated the resistance profile, presence of β-lactamases encoding genes and the clonal relationships in Acinetobacter baumannii isolated from Brazilian soils.
Methods And Results:
Soil isolates of A. baumannii were subjected to antimicrobial susceptibility testing by disk diffusion and minimum inhibitory concentration methods. Different β-lactamases encoding genes were screened by PCR and the molecular typing of these isolates was performed through the multilocus sequence typing. Non-susceptibility to different antibiotics was found, since environmental isolates were classified as multidrug-resistant. The blaSHV gene was the most prevalent, followed by blaGES. All sequence types (STs) found (ST1584, ST1607, ST1608, ST1609, ST1610, ST1611 and ST1612) were described for the first time in this study.
Conclusion:
The wide variety of new alleles and new STs detected in the present study indicates a divergent population compared to studies that are carried out in the clinical environment and points to an even larger genetic diversity within the species than was anticipated.
Significance And Impact Of The Study:
A number of the environmental isolates represented multidrug-resistant strains, a phenotype that has been more commonly reported for clinical isolates of A. baumannii; the detection of several β-lactamase encoding genes in the investigated isolates is of great concern suggesting that there is a large reservoir of these resistance genes in the environment.
More Related Videos
05:06Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
Published on: January 5, 2024
07:59Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Related Concept Videos
The Soil Ecosystem
Encoding
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
Cell Specific Gene Expression
Organization of Genes
Proteins: From Genes to Degradation
Transcription is the synthesis of RNA...
Resistivity
