Related Experiment Video

Updated: Feb 11, 2026

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
07:10

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Published on: January 7, 2019

16.4K

Efficacy studies of Sclerotium rolfsii lectin on breast cancer using NOD SCID mouse model

Prajna Hegde1, Jagadeesh Narasimhappagari1, Bale M Swamy1

  • 1Department of Studies in Biochemistry, Karnatak University, Dharwad, India.

Chemical Biology & Drug Design
|April 21, 2018
PubMed

Insights

Sclerotium rolfsii lectin (SRL) shows significant potential in breast cancer therapy by inhibiting tumor growth and angiogenesis. In vitro and in vivo studies demonstrate SRL

Area of Science:

  • Biochemistry and Molecular Biology
  • Cancer Research
  • Pharmacology

Background:

  • Altered glycan expression (TF, Tn, sTn antigens) is common in carcinomas and a target for cancer therapy.
  • Sclerotium rolfsii lectin (SRL) specifically recognizes TF and its substituted forms, indicating potential therapeutic applications.
  • Previous studies have shown SRL's interaction with various cancer cells, suggesting its clinical relevance.

Purpose of the Study:

  • To investigate the in vitro and in vivo efficacy of Sclerotium rolfsii lectin (SRL) as a potential therapeutic agent for breast cancer.
  • To evaluate SRL's effects on the growth, adhesion, and angiogenesis of breast cancer cells.
  • To assess the in vivo anti-tumor activity and toxicity of SRL in a mouse xenograft model.

Main Methods:

  • In vitro studies utilized MTT assay, flow cytometry, adhesion assays, and the chick chorioallantoic membrane (CAM) assay on MDA-MB-468 breast cancer cells.
  • In vivo efficacy studies were conducted on NOD SCID mice bearing MCF-7 xenografts.
  • Binding affinity, growth inhibition (IC50), anti-angiogenesis, and anti-adhesion effects of SRL were quantified.

Main Results:

  • SRL exhibited strong binding to MDA-MB-468 cells (MFI of 85.5) and demonstrated significant growth inhibition (IC50 of 32 μg/ml at 48 hr).
  • SRL displayed anti-angiogenesis and anti-adhesion effects on fibronectin and collagen (36% and 42% inhibition at 20 μg/ml, respectively).
  • In vivo studies showed substantial tumor regression in MCF-7 xenografts (61.77% at 20 mg/kg and 75.71% at 30 mg/kg) with no observed toxicity.

Conclusions:

  • These findings strongly support the clinical potential of Sclerotium rolfsii lectin (SRL) in the treatment of breast cancer.
  • SRL demonstrates multifaceted anti-cancer properties, including direct growth inhibition, anti-angiogenesis, and anti-adhesion.
  • The lack of toxicity in vivo further enhances SRL's promise as a therapeutic candidate for breast cancer.
Keywords:
MCF-7MDA-MB-468Sclerotium rolfsii lectinbreast cancerefficacytumor regression

More Related Videos

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model09:29

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

18.9K
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis08:11

An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

Published on: August 14, 2016

32.6K

Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.6K
Self-Efficacy01:29

Self-Efficacy

Self-efficacy is the belief in one's capacity to organize and execute actions necessary to manage prospective situations. This belief significantly influences how individuals approach goals, tasks, and challenges across different domains of life.Psychological and Educational ImpactsIndividuals with strong self-efficacy are more resilient in the face of difficulties. They are more likely to adopt effective problem-solving strategies, persist through obstacles, and regulate emotions such as...
237
Typical Model Studies01:30

Typical Model Studies

Fluid mechanics model studies often utilize scaled-down systems to predict fluid behavior in full-scale environments, such as river flows, dam spillways, and structures interacting with open surfaces. Maintaining Froude number similarity in river models is crucial, as it replicates surface flow features like wave patterns and velocities.
638
Dose-Response Relationship: Potency and Efficacy01:22

Dose-Response Relationship: Potency and Efficacy

The potency of a drug is the measure of its ability to produce a biological response and can be compared by looking at the half-maximum effective concentration or EC50 values of different drugs. A lower EC50 value indicates higher potency of the drug. In the dose–response curve of two antihypertensive drugs, candesartan and irbesartan, a significant difference is observed in their EC50 values. A lower EC50 value for candesartan indicates that it is more potent than irbesartan, as it...
6.6K
Cancer02:18

Cancer

Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
54.5K
What is Cancer?02:12

What is Cancer?

Cells and tissues must meticulously coordinate their activities for the normal functioning of the human body. Therefore, they exhibit socially responsible behavior - resting, growing, dividing, differentiating, or dying - for the organism’s benefit. Cancer arises when cells divide uncontrollably and invade other tissues or organs.
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of...
14.5K