Cell wall properties in Oryza sativa influence mesophyll CO₂ conductance

Diffusion of CO₂ from the leaf intercellular air space to the site of carboxylation (g_m ) is a potential trait for increasing net rates of CO₂ assimilation (A_net ), photosynthetic efficiency, and crop productivity. Leaf anatomy plays a key role in this process; however, there are few investigations into how cell wall properties impact g_m and A_net . Online carbon isotope discrimination was used to determine g_m and A_net in Oryza sativa wild-type (WT) plants and mutants with disruptions in cell wall mixed-linkage glucan (MLG) production (CslF6 knockouts) under high- and low-light growth conditions. Cell wall thickness (T_cw ), surface area of chloroplast exposed to intercellular air spaces (S_c ), leaf dry mass per area (LMA), effective porosity, and other leaf anatomical traits were also analyzed. The g_m of CslF6 mutants decreased by 83% relative to the WT, with c. 28% of the reduction in g_m explained by S_c . Although A_net /LMA and A_net /Chl partially explained differences in A_net between genotypes, the change in cell wall properties influenced the diffusivity and availability of CO₂ . The data presented here indicate that the loss of MLG in CslF6 plants had an impact on g_m and demonstrate the importance of cell wall effective porosity and liquid path length on g_m .