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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
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Sulfatide isoform pattern in cerebrospinal fluid discriminates progressive MS from relapsing-remitting MS
Lenka Novakova1, Avadhesh Kumar Singh2, Markus Axelsson1
1Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Journal of Neurochemistry
|April 21, 2018
Summary
Cerebrospinal fluid sulfatide isoforms show potential as biomarkers for multiple sclerosis (MS) progression. Distinct sulfatide profiles in progressive MS (PMS) may help differentiate disease phenotypes.
Area of Science:
- Neuroscience
- Biochemistry
- Immunology
Background:
- Multiple sclerosis (MS) is a central nervous system inflammatory demyelinating disease.
- Cerebrospinal fluid (CSF) biomarkers, including proteins and lipids, reflect MS pathophysiology, especially in relapsing-remitting MS (RRMS).
- Sulfatide, a myelin sheath lipid, is altered in MS CSF and may be an autoimmune target.
Purpose of the Study:
- To investigate CSF sulfatide isoforms as potential biomarkers for MS.
- To differentiate between RRMS, progressive MS (PMS), and healthy donors (HD) using sulfatide profiles.
- To assess the role of sulfatide isoforms in MS disease progression.
Main Methods:
- Quantitative mass spectrometry was used to measure sulfatide isoforms in CSF.
- CSF samples were analyzed from RRMS patients, PMS patients, and healthy donors.
- Multivariate discriminant analysis was employed to analyze sulfatide patterns.
Main Results:
- Total CSF sulfatide and specific isoforms (C24:1, C26:1, C26:1-OH) were elevated in PMS patients compared to RRMS patients and HD.
- The C23:0-OH sulfatide isoform was decreased in PMS patients compared to the other groups.
- Sulfatide isoform patterns in PMS were distinct and did not overlap with RRMS or HD groups.
- Sulfatide levels did not correlate with other biomarkers or clinical parameters.
Conclusions:
- CSF sulfatide isoform levels can distinguish between MS phenotypes (PMS vs. RRMS).
- Sulfatide isoform profiles may serve as biomarkers for MS progression.
- These findings suggest a potential role for sulfatides in the pathogenesis of MS progression.
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