Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

4.0K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
4.0K
Factors Influencing Microbial Growth: pH01:29

Factors Influencing Microbial Growth: pH

1.3K
Microorganisms are classified as acidophiles, neutrophiles, or alkaliphiles based on their pH growth preferences, reflecting their adaptations to specific environments. Maintaining a stable intracellular pH is critical for macromolecular stability and enzymatic activity, which can be challenged by external pH variations.Neutrophiles, such as Escherichia coli, grow optimally between pH 5.5 and 8.0. These microorganisms inhabit neutral or slightly acidic environments and employ mechanisms like...
1.3K
Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

868
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
868
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry01:20

Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry

556
Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
556
Factors Influencing Microbial Growth: Temperature01:27

Factors Influencing Microbial Growth: Temperature

1.4K
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
1.4K
Factors Influencing Microbial Growth: Osmolarity01:28

Factors Influencing Microbial Growth: Osmolarity

961
Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
961

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular switches meet multivalency: Antimicrobial activity of multimeric peptide assemblies from the pneumococcal LytA autolysin.

International journal of biological macromolecules·2026
Same author

Solvent-Induced Stereomutation in Supramolecular Assemblies Explained by Hansen Solubility Parameters.

Journal of the American Chemical Society·2026
Same author

Stable Protein-Based G-Quadruplex-Derived Supramolecular Bioinks as Tunable ECM-Mimetic Constructs Assembled by Combining Non-Covalent and Covalent Strategies.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

The Emergence of Chirality in Time and Space: Transient Asymmetry in Supramolecular Polymers Triggered by Visible Light.

Journal of the American Chemical Society·2026
Same author

A microfluidic method for controlled generation and trapping of membraneless water-in-water droplets.

Lab on a chip·2026
Same author

Controlling the Physical Properties in Hybrid Hydrogel Networks via Tunable Supramolecular Interactions.

Macromolecules·2026
Same journal

Toward Sustainable Optics: Fully Biomass-Based Vitrimer Film for Near-Infrared Transparency.

Biomacromolecules·2026
Same journal

Poly(furfuryl alcohol) as a Surface Modifier for Cellulose Nanocrystals Reinforced HDPE Nanocomposites.

Biomacromolecules·2026
Same journal

Cutinase Adsorption to Polyester Surfaces Modulates Dissolved Oligomer Speciation during Enzymatic Depolymerization.

Biomacromolecules·2026
Same journal

Controlled Synthesis of Sulfated Glycopolypeptides as Heparin Mimics via <i>N</i>-Carboxyanhydride ROP for Anticoagulant Property.

Biomacromolecules·2026
Same journal

Ligno-Suberized Fruit Periderm as a Mechanically Reinforced Biomacromolecular Composite.

Biomacromolecules·2026
Same journal

Effect of Hydrophilic Brush Length and Hydrophobic Chain on Biodistribution of Polymethacrylate-Based Statistical Copolymers.

Biomacromolecules·2026
See all related articles

Related Experiment Video

Updated: Feb 11, 2026

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

19.3K

Supramolecular Platform Stabilizing Growth Factors.

Simone I S Hendrikse1, Sergio Spaans1, E W Meijer1

  • 1Institute for Complex Molecular Systems , Eindhoven University of Technology , P.O. Box 513 , 5600 MB Eindhoven , The Netherlands.

Biomacromolecules
|April 21, 2018
PubMed
Summary
This summary is machine-generated.

This study stabilizes growth factors like TGF-β1 using supramolecular polymers, enhancing their half-life and cellular response. This allows for lower concentrations, improving in vitro and in vivo applications.

More Related Videos

Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

10.5K
Preparing T Cell Growth Factor from Rat Splenocytes
18:27

Preparing T Cell Growth Factor from Rat Splenocytes

Published on: October 31, 2007

13.1K

Related Experiment Videos

Last Updated: Feb 11, 2026

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

19.3K
Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

10.5K
Preparing T Cell Growth Factor from Rat Splenocytes
18:27

Preparing T Cell Growth Factor from Rat Splenocytes

Published on: October 31, 2007

13.1K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Molecular Biology

Background:

  • Supraphysiological concentrations of growth factors are often needed in studies due to their inherent instability.
  • High concentrations can lead to oversaturation and adverse effects in both in vitro and in vivo settings.
  • Existing methods for growth factor stabilization are limited.

Purpose of the Study:

  • To develop a method for stabilizing growth factors using supramolecular polymers.
  • To investigate the effect of supramolecular stabilization on the half-life and bioactivity of TGF-β1 and BMP4.
  • To explore the potential of this approach for controlled release applications in hydrogels.

Main Methods:

  • Supramolecular polymers were engineered with sulfonated peptides, mimicking heparan sulfate binding.
  • Transforming growth factor-beta 1 (TGF-β1) and Bone morphogenetic protein 4 (BMP4) were noncovalently bound to the polymers.
  • Luciferase assays were used to measure cellular response and assess growth factor activity.

Main Results:

  • Stabilized TGF-β1 and BMP4 exhibited a significantly prolonged half-life compared to free growth factors.
  • The supramolecular assembly maintained high bioactivity of TGF-β1, as evidenced by robust cellular response.
  • Lower concentrations of growth factors were effective when presented via the supramolecular polymer system.

Conclusions:

  • Supramolecular polymers offer an effective strategy for stabilizing growth factors like TGF-β1 and BMP4.
  • This stabilization enhances growth factor half-life and preserves bioactivity, enabling reduced effective concentrations.
  • The developed approach holds promise for advanced applications in tissue engineering and controlled drug delivery systems.