Related Experiment Video

Updated: Aug 9, 2026

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells
08:10

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

Published on: May 24, 2011

Liver-associated macrophage precursor cells proliferate under impairment of regular hemopoiesis

T Decker1, M L Lohmann-Matthes, M Baccarini

  • 1Department of Immunobiology, Fraunhofer Institute of Toxicology, Hannover, FRG.

European Journal of Immunology
|May 1, 1988
PubMed

Insights

Immune cells called macrophage precursor (M phi P) cells reside in normal mouse livers. Pyran copolymer treatment boosts M phi P cells and enhances their immune functions, suggesting the liver

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Previous studies identified immature macrophage precursor (M phi P) cells in pretreated mouse spleen and liver.
  • This study investigates M phi P cells in the livers of normal, untreated mice.

Purpose of the Study:

  • To demonstrate the presence and characteristics of M phi P cells in normal mouse livers.
  • To investigate the effect of pyran copolymer on liver M phi P cells and their functions.
  • To explore the hematopoietic potential of liver non-parenchymal cells (LNPC).

Main Methods:

  • Utilized proliferation assays and flow cytometry to identify M phi P cells based on CSF-1 response and F4/80 antigen expression.
  • Administered pyran copolymer to normal and bone marrow-irradiated, splenectomized mice.
  • Assessed M phi P cell numbers, CSF-1 responsiveness, natural killer activity, and Candida albicans cytostasis.
  • Conducted transplantation experiments using LNPC from treated mice.

Main Results:

  • Macrophage precursor (M phi P) cells were identified in the livers of normal mice.
  • Pyran copolymer significantly increased M phi P cell numbers and enhanced their proliferative response to CSF-1.
  • Treated LNPC exhibited augmented natural killer activity and cytostasis against Candida albicans.
  • LNPC transplantation supported the survival of lethally irradiated mice, indicating hematopoietic potential.

Conclusions:

  • The liver harbors endogenous macrophage precursor (M phi P) cells in normal mice.
  • Pyran copolymer stimulates liver M phi P cells and enhances their immune functions.
  • The liver possesses hematopoietic stem cells, contributing to hematopoiesis, especially during impaired bone marrow function.

More Related Videos

Isolation of CD133+ Liver Stem Cells for Clonal Expansion12:06

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Published on: October 10, 2011

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry08:59

Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

Published on: July 17, 2017

Related Concept Videos

Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
Production of Formed Elements01:34

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...