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Updated: Feb 11, 2026

Assessment of Physical Activity Intensity with Accelerometers and Oxygen Consumption
Published on: June 20, 2025
Variability and reliability study of overall physical activity and activity intensity levels using
Lina Jaeschke1, Astrid Steinbrecher1, Stephanie Jeran1
1Molecular Epidemiology Group, Max Delbrueck Center for Molecular Medicine in the Helmholtz Association (MDC), Robert-Roessle-Strasse 10, 13125, Berlin, Germany.
24-hour accelerometry reveals significant daily variability in physical activity (PA). One week of monitoring is sufficient for reliably estimating habitual PA, with minimal influence from the day of the week.
Area of Science:
- Biomedical Engineering
- Epidemiology
- Public Health
Background:
- Objective physical activity (PA) assessment using 24-hour accelerometry is crucial for observational studies.
- Key parameters like PA variability, required observation duration, and reliability remain unclear.
Purpose of the Study:
- To assess the variability of 24-hour physical activity (PA) using accelerometry.
- To determine the optimal observational time needed for reliable estimation of habitual PA.
- To evaluate the reliability of PA measurements across different days and days of the week.
Main Methods:
- Utilized triaxial accelerometers (ActiGraph GT3X+) to measure 24-hour PA in 50 participants over 2 weeks.
- Employed linear mixed-effects and random effects models to quantify PA variability.
- Calculated the necessary number of assessment days and PA reliability using intraclass correlation coefficients.
Main Results:
- Between- and within-person variance explained 34.4-45.5% and 54.5-65.6% of total PA variance, respectively.
- PA levels showed slight decreases in the initial 3 days and minor differences across days of the week (highest on Wednesday/Friday, lowest on Sunday/Monday).
- An average of 1 week of assessment was required for habitual PA estimation, with good week-to-week reliability (ICC range: 0.68-0.82).
Conclusions:
- Individual physical activity (PA) assessed via 24-hour accelerometry exhibits significant daily variability.
- The day of assessment or day of the week minimally impacts overall PA variance.
- A minimum of 1 week of accelerometry data is recommended for reliable estimation of habitual PA.
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