A versatile polar-embedded polyphenyl phase for multimodal separation in liquid chromatography.

Area of Science:

• Analytical Chemistry
• Chromatography

Background:

• Development of novel stationary phases is crucial for improving chromatographic separation.
• Existing phases may lack selectivity for specific analyte classes like isomers and congeners.

Purpose of the Study:

• To synthesize and characterize a new polar-embedded aromatic stationary phase.
• To evaluate its chromatographic performance compared to existing phases.
• To investigate the relationship between surface chemistry and selectivity.

Main Methods:

• Covalent attachment of p-biphenylacetamide silane to silica spheres.
• Chromatographic analysis of diverse analytes including isomers and congeners.
• Comparative study of retention behavior with alkylamide and octadecyl phases.

Main Results:

• The new phase exhibited unique benzenoid affinity and enhanced aromatic selectivity.
• Ligand properties influenced chromatographic selectivity for various congeners.
• The phase demonstrated good water-wettability.

Conclusions:

• The developed polar-embedded aromatic phase offers superior selectivity for aromatic compounds.
• Its unique properties allow application in normal phase, reversed-phase, and per-aqueous modes.
• This advancement provides a versatile tool for complex mixture analysis.