Related Experiment Video
Updated: Feb 11, 2026

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
New insight into the method of posttransformational vector amplification (PTVA) in Pichia pastoris
Liangcheng Jiao1, Qinghua Zhou1, Wu Liu1
1Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Abstract:
Posttransformational vector amplification (PTVA) is widely used to enrich the gene-copy number in Pichia pastoris. We engineered two test strains for PTVA studies and demonstrate that the PTVA process results in the amplification of a fragment with the resistant gene flanked by two homologous arms instead of the entire vector.
Related Concept Videos
Vector Algebra: Graphical Method
We use the laws of geometry to construct resultant vectors, followed by trigonometry to find vector magnitudes and directions. For a geometric construction of the sum of two vectors in a plane, we follow the parallelogram rule. Suppose two vectors are at arbitrary positions. Translate either one of...
Vector Algebra: Method of Components
In many applications, the magnitudes and directions of...
Vectors
RACE - Rapid Amplification of cDNA Ends
Acceleration Vectors
Vector Operations
A vector multiplied by a scalar value is called scalar multiplication. The result obtained is a new vector with a different magnitude. If the scalar is positive, the direction of the vector remains the same, but if it is negative, the direction of the vector is reversed. For example, the product of the mass and velocity yields the momentum.

