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Long non-protein coding RNA DANCR functions as a competing endogenous RNA to regulate osteoarthritis progression via

Xiaochen Fan1, Jishan Yuan1, Jun Xie1

  • 1Department of Orthopaedics, Affiliated Zhenjiang First Hospital of Jiangsu University, Zhenjiang 212002, China.

Biochemical and Biophysical Research Communications
|April 22, 2018
PubMed

Insights

Long noncoding RNA (lncRNA) DANCR is upregulated in osteoarthritis (OA). Inhibiting DANCR reduces OA chondrocyte proliferation and increases apoptosis via the miR-577/SphK2 pathway, suggesting DANCR as a potential OA therapeutic target.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Long noncoding RNAs (lncRNAs) play critical roles in various diseases.
  • Osteoarthritis (OA) is a debilitating joint disease with complex molecular underpinnings.
  • Understanding the role of specific lncRNAs in OA pathogenesis is crucial for developing new treatments.

Purpose of the Study:

  • To investigate the role of differentiation antagonizing non-protein coding RNA (DANCR) in osteoarthritis.
  • To elucidate the molecular mechanisms by which DANCR influences OA chondrocytes.
  • To explore the potential of DANCR as a therapeutic target for OA.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to measure DANCR expression in OA cartilage.
  • Cell counting kit 8 (CCK-8) assay to assess chondrocyte viability.
  • Flow cytometry to evaluate chondrocyte apoptosis.
  • Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays to confirm interactions between DANCR, miR-577, and SphK2.

Main Results:

  • DANCR expression was significantly upregulated in OA cartilage samples compared to controls.
  • Inhibition of DANCR suppressed the proliferation of OA chondrocytes and induced apoptosis.
  • DANCR functions as a competing endogenous RNA (ceRNA) by sponging miR-577.
  • miR-577 targets the mRNA of Sphingosine kinase 2 (SphK2), thereby regulating OA chondrocyte survival.

Conclusions:

  • lncRNA DANCR promotes OA chondrocyte proliferation and inhibits apoptosis through the miR-577/SphK2 axis.
  • The findings identify a novel regulatory pathway involving DANCR, miR-577, and SphK2 in OA.
  • lncRNA DANCR represents a potential therapeutic target for osteoarthritis treatment.
Keywords:
OsteoarthritisSphK2ceRNAlncRNA DANCRmiR-577

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