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Nrf2 protects against acute lung injury and inflammation by modulating TLR4 and Akt signaling

Jia Yan1, Jingjie Li1, Lei Zhang1

  • 1Department of Anesthesiology, Shanghai JiaoTong University School of Medicine, Center for Specialty Strategy Research of Shanghai Jiao Tong University China Hospital Development Institute, Shanghai Ninth People's Hospital, No. 639 Zhizaoju Road, Shanghai 200011, China.

Free Radical Biology & Medicine
|April 22, 2018
PubMed

Insights

Nuclear factor erythroid 2-related factor 2 (Nrf2) deficiency worsens lung injury by increasing toll-like receptor 4 (TLR4) and impairing Akt signaling. Targeting the Nrf2/TLR4/Akt axis may treat inflammation-associated lung damage.

Area of Science:

  • Cellular and Molecular Biology
  • Immunology
  • Pathology

Background:

  • Lung injury is a significant clinical issue linked to systemic inflammation.
  • Intestinal ischemia-reperfusion (I/R) injury can trigger lung damage.
  • The roles of nuclear factor erythroid 2-related factor 2 (Nrf2) and toll-like receptor 4 (TLR4) in this process require elucidation.

Purpose of the Study:

  • To investigate the involvement of Nrf2 and TLR4 in intestinal I/R-induced lung injury.
  • To explore the molecular mechanisms underlying Nrf2's protective effects in lung injury models.

Main Methods:

  • In vivo studies using Nrf2-knockout mice and I/R models.
  • In vitro experiments with mouse lung epithelial cells subjected to oxygen and glucose deprivation/reperfusion (OGD/Rep).
  • Techniques included western blotting, immunohistochemistry, TUNEL assay, and analysis of bronchoalveolar lavage fluid.

Main Results:

  • Nrf2 deficiency exacerbated I/R-induced lung injury, increasing apoptosis, inflammation, and autophagy.
  • Nrf2 deficiency led to TLR4 upregulation and Akt inactivation.
  • Nrf2 protected lung cells by modulating TLR4 and Akt signaling pathways.

Conclusions:

  • The Nrf2/TLR4/Akt axis is crucial in inflammation-associated lung damage.
  • Nrf2 plays a protective role against I/R-induced lung injury.
  • This axis represents a potential therapeutic target for lung injury treatment.
Keywords:
Acute lung injuryAktNuclear factor erythroid 2-related factor 2Toll-like receptor

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