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Related Experiment Video

Updated: Feb 11, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

A Versatile Vector Toolkit for Functional Analysis of Rice Genes.

Feng He^1,2, Fan Zhang¹, Wenxian Sun²

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Rice (New York, N.Y.)

|April 22, 2018

Summary

Researchers developed a versatile vector toolkit for rice functional genomics. This toolkit accelerates the study of gene function in rice (Oryza sativa), a crucial crop and model plant.

Keywords:

Binary vector CRISPR/Cas9 Oryza sativa Transient expression Vector toolkit

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Published on: October 6, 2017

Area of Science:

Plant Molecular Biology
Genomics
Crop Science

Background:

Rice (Oryza sativa) is a staple food for over half the global population.
It serves as a model organism for monocotyledonous plant molecular biology.
Despite genome sequencing, many rice gene functions remain uncharacterized.

Purpose of the Study:

To develop an efficient and versatile vector toolkit for rice functional genomics.
To facilitate high-throughput analysis of rice gene functions.

Main Methods:

Development of a 42-vector toolkit for transient and stable expression in rice.
Incorporation of a universal multiple cloning site for easy gene transfer.
Application of vectors for protein localization, interaction, overexpression, and CRISPR/Cas9 gene editing.

Main Results:

Successful development and validation of a comprehensive vector toolkit.
Demonstrated utility in diverse functional genomics applications.
Enabled convenient transfer of over 99% of rice coding sequences.

Conclusions:

The developed vector toolkit offers a highly efficient and high-throughput solution for rice gene functional analysis.
This resource will significantly advance our understanding of rice biology and genetics.