Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

5.1K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.1K
Phylogenetic Trees03:21

Phylogenetic Trees

49.9K
Phylogenetic trees come in many forms. It matters in which sequence the organisms are arranged from the bottom to the top of the tree, but the branches can rotate at their nodes without altering the information. The lines connecting individual nodes can be straight, angled, or even curved.
49.9K
Facilitated Transport01:19

Facilitated Transport

151.0K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
151.0K
Facilitated Transport01:19

Facilitated Transport

18.8K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
18.8K
Social Facilitation01:04

Social Facilitation

36.6K
Not all intergroup interactions lead to negative outcomes. Sometimes, being in a group situation can improve performance. Social facilitation occurs when an individual performs better when an audience is watching than when the individual performs the behavior alone. This typically occurs when people are performing a task for which they are skilled.
36.6K
Facilitated Diffusion01:16

Facilitated Diffusion

1.3K
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic disruption of leucine rich repeat transmembrane protein 4 like 1 induces a pro-social behavioural phenotype in zebrafish.

Neurobiology of disease·2025
Same author

Rapid on-site universal vertebrate species identification via multi-barcode nanopore sequencing.

PloS one·2025
Same author

Whole-genome sequences provide insights into the formation and adaptation of human populations in the Himalayas.

Current biology : CB·2025
Same author

UYSD: a novel data repository accessible via public website for worldwide population frequencies of Y-SNP haplogroups.

European journal of human genetics : EJHG·2025
Same author

Multiple origins and phenotypic implications of an extended human pseudoautosomal region shown by analysis of the UK Biobank.

American journal of human genetics·2025
Same author

Evaluating genome-wide and targeted forensic sequencing approaches to kinship determination.

Forensic science international. Genetics·2025

Related Experiment Video

Updated: Feb 11, 2026

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

19.7K

A phylogenetic framework facilitates Y-STR variant discovery and classification via massively parallel sequencing.

Tunde I Huszar1, Mark A Jobling1, Jon H Wetton1

  • 1Department of Genetics & Genome Biology, University of Leicester, University Road, Leicester LE1 7RH, UK.

Forensic Science International. Genetics
|April 22, 2018
PubMed
Summary

Massively parallel sequencing (MPS) reveals significantly more Y-chromosome short tandem repeat (Y-STR) diversity than previously detected. This advanced method enhances Y-STR analysis and aids in standardizing nomenclature within phylogenetic contexts.

Keywords:
Massively parallel sequencingPPY23PowerSeq systemRepeat pattern variation (RPV)Single nucleotide polymorphism (SNP)Y-STRs

More Related Videos

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

20.0K
Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

22.4K

Related Experiment Videos

Last Updated: Feb 11, 2026

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

19.7K
Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

20.0K
Massively Parallel Reporter Assays in Cultured Mammalian Cells
11:03

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

22.4K

Area of Science:

  • Forensic genetics and human population studies.
  • Molecular biology and genomics.
  • Y-chromosome analysis and phylogenetics.

Background:

  • Y-chromosome short tandem repeats (Y-STRs) are valuable genetic markers due to their linkage and phylogenetic framework provided by single nucleotide polymorphisms (SNPs).
  • Previous Y-STR analysis methods, like capillary electrophoresis (CE), have limitations in detecting the full spectrum of allelic diversity.

Purpose of the Study:

  • To utilize massively parallel sequencing (MPS) to comprehensively analyze Y-STR diversity within the PowerPlex® Y23 (PPY23) system.
  • To compare MPS results with existing CE data and identify novel Y-STR variants and their phylogenetic significance.

Main Methods:

  • Analysis of 23 Y-STRs in 100 diverse Y chromosomes using MPS.
  • Characterization of alleles, including duplications and potential somatic mutations.
  • Phylogenetic analysis of Y-STR variants in the context of known Y chromosome haplogroups.

Main Results:

  • MPS identified 2311 alleles with 99.83% concordance to CE data.
  • A 58% increase in detectable alleles was observed, with 267 distinct sequence-based alleles identified, including 60 novel Y-STR variants.
  • Novel variations were found in repeat and flanking regions, including repeat pattern variants (RPVs) and SNP/indel differences, with some showing phylogenetic associations and others exhibiting recurrence.

Conclusions:

  • MPS significantly expands the detection of Y-STR diversity within the PPY23 marker set.
  • The study demonstrates high concordance between MPS and CE, supporting MPS as a robust method for Y-STR analysis.
  • Findings facilitate Y-STR nomenclature standardization and provide a phylogenetic context for observed sequence variants.