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Updated: Feb 11, 2026

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
Published on: July 13, 2016
Colloidal gold-based immunochromatographic strip assay for the rapid detection of three natural estrogens in milk
Zhongxing Wang1, Lingling Guo1, Liqiang Liu1
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Abstract:
In this study, we developed highly sensitive and specific monoclonal antibodies (mAbs) against estrone (E1), 17β-estradiol (17β-E2), and estriol (E3). The half-maximal inhibitory concentration values of anti-E1, anti-17β-E2, and anti-E3 mAbs were 0.46, 0.36, and 0.39 ng/mL, respectively, based on competitive enzyme-linked immunosorbent assay (ic-ELISA) results. A rapid colloidal gold-based immunoassay strip assay was developed for the determination of E1, 17β-E2, and E3 residues in milk samples. The assay had a visual cut-off value of 5 ng/mL, and required 10 min to assess with the naked eye. The results obtained from the immunochromatographic strip assay were consistent with those obtained from ic-ELISA and gas chromatography-mass spectrometry. The immunochromatographic strip assay is useful and rapid for the detection of E1, 17β-E2, and E3 in milk.
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