Related Experiment Video

Updated: Feb 11, 2026

Breath Collection from Children for Disease Biomarker Discovery
06:09

Breath Collection from Children for Disease Biomarker Discovery

Published on: February 14, 2019

7.4K

Comparison of two exhaled biomarkers in children with and without sleep disordered breathing

Mario Barreto1, Paolo Montuschi2, Melania Evangelisti1

  • 1Pediatric Unit Sant'Andrea Hospital, NESMOS Department, Faculty of Medicine and Psychology, "Sapienza" University, Rome, Italy.

Sleep Medicine
|April 23, 2018
PubMed

Insights

8-isoprostane (8-IsoP) in exhaled breath condensate effectively identifies obstructive sleep apnea (OSA) in children. This biomarker correlates with disease severity and predicts OSA, unlike nitric oxide levels.

Area of Science:

  • Pediatric Pulmonology
  • Sleep Medicine
  • Biomarker Research

Background:

  • Airway inflammation and oxidative stress are implicated in pediatric sleep disordered breathing (SDB).
  • Understanding biomarkers for SDB severity is crucial for diagnosis and management.

Purpose of the Study:

  • To measure 8-isoprostane (8-IsoP) in exhaled breath condensate (EBC) and fractional exhaled nitric oxide (FeNO) in children with SDB.
  • To assess the relationship between these biomarkers, SDB severity, and overnight changes.

Main Methods:

  • Exhaled measurements (EBC for 8-IsoP, FeNO) were performed in 46 children with SDB and 20 healthy controls.
  • SDB severity was assessed using polysomnography, categorizing participants into primary snoring (PS) and obstructive sleep apnea (OSA).

Main Results:

  • Children with OSA showed higher 8-IsoP levels in EBC compared to PS patients and controls.
  • 8-IsoP levels correlated with apnea-hypopnea index (AHI) and oxygen saturation (SaO2), and accurately predicted OSA.
  • FeNO levels did not correlate with disease severity, though they increased in mild OSA patients overnight.

Conclusions:

  • 8-isoprostane in EBC is a valuable biomarker for distinguishing OSA from PS and healthy controls in children.
  • 8-IsoP levels reflect SDB severity and can predict OSA, offering a potential diagnostic tool.
Abstract

Keywords:
BiomarkersChildrenInflammationSleep disordered breathingSnoring

More Related Videos

Fast and Accurate Exhaled Breath Ammonia Measurement06:27

Fast and Accurate Exhaled Breath Ammonia Measurement

Published on: June 11, 2014

14.0K
Author Spotlight: Unveiling the Connection Between Sleep Disorders and Cognitive Symptoms in Depression04:33

Author Spotlight: Unveiling the Connection Between Sleep Disorders and Cognitive Symptoms in Depression

Published on: April 26, 2024

1.5K

Related Concept Videos

Breathing01:05

Breathing

The process of breathing, inhaling and exhaling, involves the coordinated movement of the chest wall, the lungs, and the muscles that move them. Two muscle groups with important roles in breathing are the diaphragm, located directly below the lungs, and the intercostal muscles, which lie between the ribs. When the diaphragm contracts, it moves downward, increasing the volume of the thoracic cavity and creating more room for the lungs to expand. When the intercostal muscles contract, the ribs...
64.7K
Substance Use Disorders Affecting Sleep01:24

Substance Use Disorders Affecting Sleep

Substance use disorders involve a pattern of using drugs more extensively than intended and continuing use despite harmful consequences. This includes legal substances like alcohol and nicotine, as well as illegal drugs. These disorders often involve both physical and psychological dependence, reflecting compulsive use of substances that significantly alter thoughts, feelings, and behaviors, contributing to a major public health issue.
Understanding the concepts of physical dependence,...
462
REM Sleep Behavior Disorder01:15

REM Sleep Behavior Disorder

REM Sleep Behavior Disorder (RBD) is a sleep disorder characterized by the absence of muscle paralysis that normally occurs during the REM phase of sleep. This absence allows individuals to physically act out their dreams, which are often vivid and disturbing. Common behaviors exhibited during episodes include kicking, punching, and yelling. These actions can be dangerous, potentially leading to injuries for the person with RBD or their bed partner.
RBD is significantly associated with...
1.8K
Pulmonary Cycle: Exhalation01:17

Pulmonary Cycle: Exhalation

In terms of human respiration, the act of expelling air, known as exhalation (or expiration), operates on the principle of pressure gradients. During expiration, the pressure within the lungs exceeds that of the surrounding atmosphere. Under normal conditions, quiet breathing involves passive exhalation and is free of muscular contractions. This is because the exhalation process is driven by the natural elastic recoil of the lungs and chest wall, both of which have an inherent tendency to...
3.7K
Insufficient Sleep and Sleep Deprivation01:13

Insufficient Sleep and Sleep Deprivation

Insufficient sleep refers to not getting the recommended amount of sleep for optimal functioning, even if it's just slightly less than needed. Sleep insufficiency may occur due to lifestyle choices, such as staying up late for social events or work, resulting in routinely getting less sleep than required. For example, consistently sleeping 6 hours when the body needs 7-9 hours can lead to cumulative effects on health and well-being.
Sleep deprivation is a more severe form of sleep loss...
962
The Sense of Self: Reflected Self-Appraisal and Social Comparison02:57

The Sense of Self: Reflected Self-Appraisal and Social Comparison

According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
56.1K