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Updated: Jul 10, 2026
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
Published on: March 3, 2015
Immunocytochemical localization of the Ca2+-ATPase polypeptide in human platelets
1Department of Anatomical Sciences, University of Louisville School of Medicine, KY 40292.
Abstract:
Specific polyclonal antibodies raised against purified human platelet Ca2+-ATPase were used with protein A-gold immunocytochemistry to localize this protein in human platelets. Immunolabeling specifically detected Ca2+-ATPase over the surface connected membrane system (SCS) in sections of paraformaldehyde-fixed, Lowicryl-embedded platelets. The maximum density of label, determined by quantitative morphometric techniques, was observed over electron-dense regions within the SCS which may represent specialized structures for uptake and release of Ca2+. Less intense immunolabeling was observed over cytosol and may represent localization over the dense tubular system (DTS) which was not readily visualized under the processing procedures employed.
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