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Updated: Feb 11, 2026

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EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples
Published on: March 28, 2015
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Full Scale Drinking Water System Decontamination at the Water Security Test Bed
Jeffrey Szabo1, John Hall2, Steve Reese3
1Environmental Engineer, U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., (MS NG-16), Cincinnati, OH 45268, 513-487-2823.
Summary
The EPA
Area of Science:
- Environmental Science
- Water Quality
- Microbiology
Background:
- The U.S. Environmental Protection Agency (EPA) established the Water Security Test Bed (WSTB) facility, a full-scale drinking water distribution system.
- The WSTB was developed in collaboration with Idaho National Laboratory (INL) to evaluate water infrastructure decontamination technologies.
- It supports research on real-time sensors, mobile water treatment, and premise plumbing decontamination.
Purpose of the Study:
- To assess the effectiveness of decontamination methods for Bacillus globigii (BG) spores and Bakken crude oil in a simulated drinking water distribution system.
- To evaluate the removal of contaminants from bulk water and pipe surfaces.
- To inform water systems on effective response methodologies for contamination incidents.
Main Methods:
- Full-scale testing within the Water Security Test Bed (WSTB) facility.
- Decontamination experiments using Bacillus globigii (BG) spores, a surrogate for Bacillus anthracis.
- Decontamination experiments using Bakken crude oil, focusing on soluble components and persistent residues.
- Evaluation of flushing and chlorination as decontamination techniques.
Main Results:
- Flushing and chlorination effectively removed the majority of BG spores from the bulk water.
- BG spores persisted on pipe wall coupons even after decontamination attempts.
- Soluble components of Bakken crude oil were removed by flushing.
- Oil residues remained in appliances such as dishwashers and refrigerator water dispensers.
Conclusions:
- Standard decontamination methods show limitations in fully removing microbial surrogates and crude oil from all parts of a water distribution system.
- Further research is needed to address persistent contaminants on pipe surfaces and within appliances.
- The WSTB facility provides a critical platform for risk-free testing of water contamination response strategies.
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