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Updated: Feb 11, 2026

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
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A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

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Replication study: androgen receptor splice variants determine taxane sensitivity in prostate cancer

Xiaochuan Shan1, Gwenn Danet-Desnoyers1, Fraser Aird2

  • 1Stem Cell and Xenograft Core, Perelman School of Medicine, Philadelphia, PA, USA.

Peerj
|April 24, 2018
PubMed

Insights

Replication experiments showed docetaxel did not affect prostate cancer xenograft growth. Researchers could not detect androgen receptor variant 7 (ARv7) in LuCaP 23.1, differing from the original study.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Prostate cancer treatment sensitivity is linked to androgen receptor (AR) splice variants.
  • Previous studies suggested AR variants influence response to taxane chemotherapy.

Purpose of the Study:

  • To replicate experiments investigating the role of AR splice variants in taxane sensitivity in prostate cancer.
  • To validate findings from Thadani-Mulero et al., 2014.

Main Methods:

  • Tumor xenografts (LuCaP 86.2 and LuCaP 23.1) were treated with docetaxel.
  • AR expression and variant presence were assessed.
  • A meta-analysis of results was performed.

Main Results:

  • Docetaxel treatment did not significantly affect the growth of LuCaP 86.2 or LuCaP 23.1 xenografts.
  • LuCaP 23.1 xenografts exhibited slower growth than reported in the original study.
  • Androgen receptor variant 7 (ARv7) was not detected in LuCaP 23.1 xenografts, despite using the original antibody and a positive control.

Conclusions:

  • The replication study did not confirm the taxane sensitivity conferred by AR splice variants as reported previously.
  • Discrepancies in xenograft growth and ARv7 detection highlight potential reproducibility challenges in this research area.
Keywords:
Androgen receptor variantsCastration resistant prostate cancerDocetaxelMethodologyPCFMFRI

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