Related Experiment Video

Updated: Feb 11, 2026

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression
09:51

Generation of Stable Human Cell Lines with Tetracycline-inducible Tet-on shRNA or cDNA Expression

Published on: March 5, 2013

36.1K

Establishment and Characterization of a High and Stable Porcine CD163-Expressing MARC-145 Cell Line

Xiangju Wu1,2, Jing Qi2, Xiaoyan Cong2

  • 1Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.

Biomed Research International
|April 24, 2018
PubMed

Insights

A new cell line expressing porcine CD163 (pCD163) enhances porcine reproductive and respiratory syndrome virus (PRRSV) infection. This improved cell line increases PRRSV isolation rates and vaccine propagation efficiency for better disease management.

Area of Science:

  • Veterinary Virology
  • Cell Biology
  • Molecular Virology

Background:

  • Porcine reproductive and respiratory syndrome virus (PRRSV) isolation and identification are crucial for disease management.
  • MARC-145 cells are standard for PRRSV research but have limitations, including low virus yield.
  • Porcine CD163 (pCD163) acts as a cellular receptor mediating PRRSV infection in non-permissive cells.

Purpose of the Study:

  • To establish a stable cell line with high porcine CD163 expression (pCD163-MARC) to enhance PRRSV susceptibility.
  • To evaluate the utility of the pCD163-MARC cell line for PRRSV isolation and propagation.

Main Methods:

  • Generation of a MARC-145 cell line stably expressing porcine CD163 (pCD163).
  • Validation of pCD163 expression using indirect immunofluorescence assay (IFA) and Western blotting.
  • Assessment of PRRSV propagation efficiency and isolation rates in pCD163-MARC cells compared to standard MARC-145 cells.

Main Results:

  • The pCD163-MARC cell line demonstrated significantly higher pCD163 expression than parental MARC-145 cells.
  • PRRSV propagation was significantly enhanced in the pCD163-MARC cell line.
  • The pCD163-MARC cell line exhibited a higher isolation rate for clinical PRRSV samples and more efficient propagation of live attenuated PRRS vaccine strains.

Conclusions:

  • The developed pCD163-MARC cell line offers increased susceptibility to PRRSV infection.
  • This novel cell line serves as a valuable tool for improved PRRSV propagation and research.
  • Enhanced PRRSV propagation and isolation capabilities can aid in PRRSV disease management and vaccine development.

More Related Videos

Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids09:43

Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids

Published on: October 14, 2019

6.9K
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity07:32

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Published on: April 13, 2021

3.7K

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
197.0K
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.7K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K