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Related Concept Videos

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Related Experiment Video

Updated: Feb 11, 2026

Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography
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In Situ Heart Isolation Featuring Closed Loop Recirculation: The Gold Standard for Optimum Cardiac Gene Transfer?

Michael G Katz1, Anthony S Fargnoli1, Roger J Hajjar1

  • 1Cardiovascular Research Center, Department of Cardiology, Icahn School of Medicine at Mount Sinai, New York, USA.

Advanced Techniques in Biology & Medicine
|April 24, 2018
PubMed
Summary
This summary is machine-generated.

Molecular cardiac surgery with recirculating delivery (MCARD) enhances cardiac gene therapy by isolating the heart and recirculating vectors. This surgical approach improves gene delivery efficiency and minimizes side effects for better therapeutic outcomes.

Keywords:
Cardiac isolationGene therapyMolecular cardiac surgery with recirculating delivery

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Area of Science:

  • Cardiovascular Medicine
  • Gene Therapy
  • Surgical Innovation

Background:

  • Cardiac gene therapy holds promise but faces challenges in efficient and targeted delivery.
  • Current methods struggle with vector contact time, cellular uptake, and global gene distribution.
  • Minimizing collateral effects and maximizing therapeutic efficacy remain critical hurdles.

Purpose of the Study:

  • To introduce and evaluate a novel surgical approach for enhanced cardiac gene delivery.
  • To address limitations in current gene delivery methods by optimizing vector circulation and cardiac targeting.
  • To investigate the potential of molecular cardiac surgery with recirculating delivery (MCARD) for improved gene transduction efficiency.

Main Methods:

  • Development of the molecular cardiac surgery with recirculating delivery (MCARD) system.
  • Isolation of the heart from systemic circulation using cardiopulmonary bypass.
  • Implementation of a "closed loop" model for multiple vector passes through the coronary vasculature.
  • Removal of residual vector post-delivery to minimize off-target expression.

Main Results:

  • MCARD facilitates cardiac-selective gene transfer by isolating the heart.
  • Multiple vector recirculation increases vector contact time within the coronary vasculature.
  • The surgical approach enhances vector transduction efficiency in cardiac tissues.
  • Isolation and vector removal minimize collateral gene expression in non-target organs.

Conclusions:

  • MCARD represents a promising surgical strategy to overcome key limitations in cardiac gene delivery.
  • This approach offers improved efficiency, targeting, and safety for therapeutic gene transfer to the heart.
  • MCARD has the potential to significantly advance clinical applications of cardiac gene therapy.