Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

24.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.3K
MicroRNAs01:22

MicroRNAs

4.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.1K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.3K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.3K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

26.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.5K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Epigenetic Regulation01:46

Epigenetic Regulation

33.9K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrophilic interaction chromatography and tandem mass spectrometry for the characterization of methylated oligoribonucleotides.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Bat Dicer antiviral role and subcellular localization differ upon alphavirus infection in two distinct species.

PLoS pathogens·2025
Same author

[Cellular RNA-binding proteins during viral infection: known factors and new approaches to identify novel targets].

Medecine sciences : M/S·2025
Same author

Extensive longevity and DNA virus-driven adaptation in nearctic <i>Myotis</i> bats.

bioRxiv : the preprint server for biology·2024
Same author

Beyond RNAi: How the Dicer protein modulates the antiviral innate immune response in mammalian cells: Mammalian Dicer could regulate the innate immune response in an RNAi-independent manner as a result of losing long dsRNA processive activity.

BioEssays : news and reviews in molecular, cellular and developmental biology·2024
Same author

Direct Zeptomole Detection of RNA Biomarkers by Ultrabright Fluorescent Nanoparticles on Magnetic Beads.

Small (Weinheim an der Bergstrasse, Germany)·2024

Related Experiment Video

Updated: Feb 11, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

8.0K

Regulation of primary microRNA processing.

Antoine Creugny1, Aurélie Fender1, Sébastien Pfeffer1

  • 1Architecture and Reactivity of RNA, Institut de Biologie Moléculaire et Cellulaire du CNRS, Université de Strasbourg, France.

FEBS Letters
|April 24, 2018
PubMed
Summary

MicroRNAs (miRNAs) are small RNAs regulating biological processes. This review focuses on the nuclear-initiated maturation of miRNAs and its dynamic regulation for cellular homeostasis.

Keywords:
DroshabiogenesismicroRNAregulation

More Related Videos

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

9.0K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K

Related Experiment Videos

Last Updated: Feb 11, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

8.0K
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

9.0K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial regulatory molecules involved in fundamental biological processes.
  • The biogenesis and function of miRNAs have been extensively studied over the last 20 years.
  • miRNA expression requires tight regulation to maintain cellular homeostasis, involving complex interactions.

Purpose of the Study:

  • To review the current understanding of the initial steps in miRNA maturation within the nucleus.
  • To explore the mechanisms governing the specific and dynamic modulation of nuclear miRNA processing.

Main Methods:

  • Literature review of recent research on miRNA biogenesis.
  • Analysis of regulatory mechanisms impacting nuclear miRNA processing.
  • Synthesis of knowledge on the dynamic modulation of miRNA maturation.

Main Results:

  • The nucleus is the primary site for the initial steps of miRNA maturation.
  • Specific proteins and RNAs dynamically regulate these early maturation events.
  • Understanding these regulations is key to comprehending miRNA's role in cellular homeostasis.

Conclusions:

  • The nuclear phase of miRNA maturation is a critical regulatory checkpoint.
  • Dynamic modulation of this step ensures appropriate miRNA levels for cellular function.
  • Further research into these mechanisms can reveal insights into cellular homeostasis and disease.