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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

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Analysis of the microRNA transcriptome of Daphnia pulex during aging

Jiabao Hu1, Chongyuan Lin1, Mengdi Liu1

  • 1School of Marine Sciences, Ningbo University, Ningbo 315211, China.

Gene
|April 24, 2018
PubMed

Insights

MicroRNAs (miRNAs) play a role in aging and reproduction in Daphnia pulex. This study sequenced miRNAs from young and old specimens, revealing significant differences in expression profiles that regulate aging and senescence.

Area of Science:

  • Developmental Biology
  • Genomics
  • Molecular Biology

Background:

  • Daphnia pulex exhibits cyclical parthenogenesis and sexual reproduction.
  • MicroRNA (miRNA) regulation of aging is a key research area.
  • Understanding miRNA's role in aging and senescence is crucial.

Purpose of the Study:

  • To investigate the role of miRNAs in regulating aging and senescence in Daphnia pulex.
  • To analyze miRNA expression profiles during the aging process.

Main Methods:

  • Sequencing of miRNA libraries from 1-day-old and 25-day-old Daphnia pulex specimens using Illumina HiSeq.
  • Bioinformatic analysis to identify differentially expressed miRNAs.
  • Quantitative real-time (qRT)-PCR validation of miRNA expression.

Main Results:

  • 1335 differentially expressed miRNAs were identified, with 127 showing statistically significant differences (P < 0.01).
  • 92 miRNAs were upregulated and 35 were downregulated between young and old specimens.
  • miRNA sequencing and qRT-PCR data were consistent, and 94 miRNAs were predicted to target 2014 genes.
  • Sixteen pathways showed significant changes (P < 0.05) during aging.

Conclusions:

  • The differential miRNA expression profile during Daphnia pulex aging provides a foundation for further research.
  • miRNAs are implicated in regulating aging, reproductive transformation, senescence, and longevity in Daphnia pulex.
Keywords:
Aging processDaphnia pulexExpression analysismiRNA

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