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Updated: Feb 11, 2026

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
Resuscitation of functional bacterial community for enhancing biodegradation of phenol under high salinity conditions
Xiaomei Su1, Yuyang Wang1, Binbing Xue1
1College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.
Abstract:
This study assumed that key degraders of functional bacterial community were prone to enter into the viable but non-culturable (VBNC) state under high saline phenolic conditions, and resuscitation-promoting factor (Rpf) could strengthen these degraders for better performances. Based on these assumptions, Rpf was used to enhance salt-tolerant phenol-degrading capability of functional populations in activated sludge. Results suggested that Rpf accelerated the start-up process during sludge domestication, and significantly enhanced salt-tolerant phenol-degrading capability. High-throughput sequencing showed that the resuscitation and stimulation functions of Rpf linked mainly to the genus Corynebacterium within the phylum Actinobacteria, and the genera Proteiniphilum and Petrimonas within the phylum Bacteroidete. These key functional populations contributed to better phenol-degrading capabilities under high salinity conditions. This study indicated that Rpf is a promising additive for improving biological treatment performance of saline phenolic wastewater.
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