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Updated: Feb 11, 2026

Generation of Fluorescent Protein Fusions in Candida Species
Published on: March 4, 2017
Single-tube classical PCR for Candida auris and Candida haemulonii identification
Laura Theill1, Catiana Dudiuk2, Soraya Morales-Lopez3
1Laboratorio de Micología y Diagnóstico Molecular - Cátedra de Parasitología y Micología - Facultad de Bioquímica y Ciencias Biológicas - Universidad Nacional del Litoral, Santa Fe, Argentina.
Background:
Candida auris and Candida haemulonii are emerging and multiresistant pathogens. C. auris has produced hospital outbreaks and is misidentified by phenotypic-based methods. The only reliable identification methods are DNA sequencing and MALDI-TOF.
Aims:
To develop a classical-PCR method capable of rapidly and accurately identify C. auris and C. haemulonii.
Methods:
A multiplex PCR was carried out in one tube that included an internal control and oligonucleotides that specifically hybridize to the ITS2 region of C. auris and C. haemulonii. The usefulness of the new method was verified by testing a collection of 50 strains of 20 different species (previously identified by ITS sequencing). The selection of species was made in order to emulate the C. auris panel used by the CDC to validate diagnostic tools. In addition, other yeast species not included in the aforementioned panel were incorporated based on reported identification errors.
Results:
The results obtained with the proposed protocol were in total agreement with those obtained by ITS sequencing.
Conclusions:
We present a PCR method able to unequivocally identify C. auris and differentiate it from C. haemulonii. It is inexpensive, fast and it could be a useful tool to reduce the chances of a C. auris outbreak.
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